苯并芘损伤胎盘绒毛组织机理及v,e防护作用的研究-妇产科学专业论文.docxVIP

英 文 摘 要  PAGE 14 The experimental research of the effort of BaP damage the placental villus and the effort of Vitamin E protect against BaP ABSTRACT Objective:1 Observing the morphologic variety of the placental villus durning 15days before pregnancy and early trimester of pregnancy exposure to the BaP; Studying the mechanism of action of damnification that BaP act on placental villus; Studying the effect of Vitamin E oppose BaP; Providing the guidelines of antenatal care and clinical research. Methods:1 Choosing of the experimental animals:150 ICR mice(100 female mice,50 male mice) were randomly divided into five groups, each with30(20 female mice,10 male mice),and raised female mice and male mice respectively. Grouping the experimental animals The control group: female mice was lavaged with BaP(2mg/kg.d); 10mg/kgVE ant-group:female mice was lavaged with BaP(2mg/kg.d)and Vitamin E(10mg/kg.d); 20mg/kgVE ant-group:female mice was lavaged with BaP(2mg/kg.d)and Vitamin E(20mg/kg.d); 40mg/kgVE ant-group:female mice was lavaged with BaP(2mg/kg.d)and Vitamin E(40mg/kg.d); The vacuity group:female mice was lavaged with olive oil in identical capacity everyday. Dealling with the experimental animals After 15days(three estrous cycles),male mice were mate with female mice(every cage included 2 female mice and 1 male mouse),The second day,Gestational age was determined based on the presence of a vaginal plug,  with the morning of detection being embryonic day 1 (d 1) of gestation,we did no treatment on male mice. Electing pregnant mice in each group. we continued lavaging BaP（2mg/kg.d）for 7 days(early trimester of pregnancy include 7 days).The mice were euthanized then detected the relevant indicators. Method of detecing related index Detecting the gene of Bax and Bcl-2 in the level of mRNA with RT-PCR in the placental villus; Detecting activity of SOD and GSH-PX with related kit; Observting the morphology change with HE; Detecting the gene of Bax and Bcl-2 in