视黄醇结合蛋白4在妊娠期糖尿病患者血清脐血及胎盘组织中表达及意义.docVIP

视黄醇结合蛋白4在妊娠期糖尿病患者血清脐血及胎盘组织中表达及意义 　　[摘要] 目的 探?妊娠期糖尿病（GDM）患者血清、脐血、胎盘组织中视黄醇结合蛋白4（RBP4）表达水平及临床意义。 方法 选取2014年1～12月浙江省嘉兴市妇幼保健院收治的40例GDM孕妇为GDM组和40例糖耐量正常的孕妇为NGT组，用逆转录聚合酶链反应检测胎盘组织中RBP4 mRNA的表达，用酶联免疫吸附试验检测孕妇血清RBP4及脐血RBP4水平，计算体重指数，检测空腹血糖、空腹胰岛素、血脂，计算胰岛素抵抗指数（HOMA-IR），并记录新生儿出生体重。 结果 两组空腹血糖、三酰甘油、HOMA-IR及新生儿出生体重比较，差异有统计学意义（P 0.05）。GDM组血清、脐血RBP4水平均高于NGT组，差异有统计学意义（P 0.05）。两组脐血RBP4水平均低于血清RBP4水平，差异有统计学意义（P 0.05）。GDM组胎盘组织中RBP4 mRNA表达水平显著高于NGT组（P 0.01）。血清RBP4水平与三酰甘油、新生儿出生体重呈正相关（r = 0.489、0.488，P 0.01）；脐血RBP4水平与新生儿出生体重呈正相关（r = 0.327，P 0.05）。 结论 GDM孕妇体内RBP4表达水平与妊娠期糖代谢异常和胎儿生长发育有关，其可能参与GDM发病。 　　[关键词] 视黄醇结合蛋白4；妊娠期糖尿病；胎盘；发病机制 　　[中图分类号] R714.25 [文献标识码] A [文章编号] 1673-7210（2016）12（b）-0080-04 　　Expression and significance of serum， cord blood and placental tissue retinol-binding protein 4 in patients with gestational diabetes mellitus 　　DONG Jinhua XUE Lili 　　Department of Obstetrics， Maternal and Child Health Care Hospital of Jiaxing City， Zhejiang Province， Jiaxing 413050， China 　　[Abstract] Objective To investigate expression and clinical significance of serum， cord blood and placental tissue retinol-binding protein （RBP） 4 in patients with gestational diabetes mellitus （GDM）. Methods 40 cases of GDM in Maternal and Child Health Care Hospital of Jiaxing City， Zhejiang Province from January to December 2014 were selected as GDM group， and 40 normal pregnant women with impaired glucose tolerance were selected as NGT group. The expression of RBP4 mRNA in placenta tissue was detected by reverse transcription polymerase chain reaction. Maternal serum and cord blood RBP4 levels were detected by enzyme-linked immunosorbent assay. Body mass index was calculated， fasting blood glucose， fasting insulin， blood lipid levels were detected， HOMA-IR was calculated， birth weight of newborn was recorded. Results Fasting blood glucose， triglyceride， HOMA-IR and birth weight of newborn between two groups were compared， with statistical differences （P 　　[Key words] Retinol-binding protein 4； Gestat