电子垃圾污染区宫内多溴联苯醚暴露对胎儿生长发育及胎盘igf-1和igfbp-3表达的影响-免疫学专业论文.docxVIP

汕头大学医学院硕士学位论文  PAGE 6 ABSTRACT Background: Guiyu as a typical e-waste contaminated area, and its extensive e-waste dismantling process had release large amounts of toxic substances into the environment, including heavy metals, polybrominated diphenyl ethers, polycyclic aromatic hydrocarbons and so on. Many studies had found high levels of polybrominated diphenyl ethers (PBDEs) have been detected in environment, organisms, population and umbilical cord blood in Guiyu. PBDEs is an important environmental pollutants, it is well documented that exposure to PBDEs can cause neural developmental toxicity, endocrine disruption, reproductive toxicity. It can through the placental barrier in pregnant woman and passed to the fetus which may directly or indirectly affect fetal growth and development. However, researches on effects of in utero exposure to PBDEs on fetal growth and development still very limited, so it is very necessary to investigate the adverse health effects of PBDEs on fetal growth and development. Objective：To investigate the association between PBDEs exposure during pregnancy and the expression of Insulin-like Growth Factor-1 (IGF-1) and Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) in fetuses, explore effects of in utero exposure to PBDEs on fetal growth and development. To provide more biological indicators for prenatal exposure to PBDEs effect on fetal growth and development. Methods: We recruited 120 pregnant women (75 from Guiyu, 45 from Chaoan) from December 2009 to June 2010, and carefully collected umbilical cord blood (UCB) and placental tissue during delivery. Questionnaires for personal data were administered. We analyzed PBDE concentrations in UCB by gas chromatography/mass spectrometry. Placental mRNA levels of IGF-1 and IGFBP-3 were determined by real-time PCR. Result: Total PBDEs concentrations were significant higher in UCB samples frome Guiyu than control (median 57.55, range 29.5725th～114.1475th ng/g lipid vs. 8.23, range 5.742