递增负荷运动对大鼠胫骨雌激素受体和转化生长因子β1表达 影响.docVIP

递增负荷运动对大鼠胫骨雌激素受体和转化生长因子β1表达 影响 　　摘 要: 目的:观察递增负荷运动后大鼠血清雌二醇、胫骨雌激素受体和转化生长因子-β1 的表达,探讨递增负荷运动后骨组织的适应机制。方法:30只SD雌性大鼠随机分为对照组 、一次性大强度运动组、长期大强度运动组。ELISA法测定血清E2含量,免疫组化测定ER和T GF-β1的表达,RT-PCR法测定ER mRNA和TGF-β1mRNA表达水平。结果:一次性大强台运 动后血E2,ER mRNA和TGF-β1mRNA水平均无显著性差异。长期大强度运动后血E2显著下 降(P0.01),ER mRNA和TGF-β1mRNA均显著下降(P0.05)。一次性运动组胫骨ER、TG F- β1的积分光密度和平均光密度组间比较均无显著性差异。长期大强度运动组ER、TGF-β1的 积分光密度显著下降(P0.05),平均光密度显著下降(P0.01)。结论:长期大强度跑 台运动可降低大鼠血清E2,下调胫骨ER和TGF-β1表达。 　　关键词:跑台运动;大鼠;胫骨 　　中图分类号:G804.7文献标识码:A文章编 号:1007-3612(2010)05-0044-04 　　 　　Expression of ER and TGF-β1 of Tibia of Rat after Heavy Treadmi ll-running 　　WANG Ping?1, LI Min?2, ZHANG Lin?3 　　(1.Department of Physical Education, Taiyuan University of Sci ence and Technology, Taiyuan 030024,Shanxi China;?2.School of Sports Sci ence, Shangqiu Normal College, Shangqiu 476000,Henan China;?3.School of 　　Sports Science Soochow University, Suzhou 215021, Jiangsu China) 　　Abstract:Aim: To investigate the expression of estrogen receptor and tra nsforming growth factor β1 of rat’s tibia after heavy treadmill running in ord er to discuss the tibia’s adaption to running. Methods: 30 female rats were ra ndomly divided into control group, a bout of heavy treadmill running group and l ong-time heavy treadmill running group. The concentration of estuarial in serumwas measured by ELISA methods. ER and TGF-β1 in protein level were detected b y immunohistochemistry methods. The expression of ER and TGF-β1 in mRNA level 　　was analyzed by semi?quantitative reverse transcription-polymerase chain reacti on technique. Results: After long?time heavy treadmill running, the concentrat ion of estuarial decreased(?p?0.01), and average optical density and integra l optical density of ER and TGF-β1 decreased(?p?0.05 or ?p?0.01), and t he estrogen receptor and transforming growth factor β1 mRNA expression decrease d (?p?0.05). Conclusion: Long?time heavy treadmill running decreases estro gen levels and inhibites the expression of ER and TGF-β1. 　　Key words