输血前应用ELISA检测梅毒螺旋体抗体临床研究.docVIP

输血前应用ELISA检测梅毒螺旋体抗体临床研究 　　[摘要] 目的 探讨输血应用酶联免疫吸附试验（ELISA）检测梅毒螺旋体抗体的临床价值。 方法 选择2007年1月～2011年1月我院住院接受输血治疗的患者5 790例作为观察对象，应用ELISA试剂盒、TPPA试剂盒进行ELISA和TPPA血清梅毒螺旋体抗体检测。 结果 5 790例入选观察对象中，应用ELISA法检测梅毒螺旋体抗体（TP-Ab）阳性50例，阳性率为0.864%；而应用TPPA法检测的梅毒螺旋体抗体阳性49例，阳性率为0.846%（49/5 790）。两种检测方法差异无统计学意义（χ2=0.643，P ＞ 0.05）。≥70岁组的应用ELISA法检测的梅毒螺旋体抗体阳性率明显低于＜70岁组，差异有统计学意义（P 0.05）。 结论 输血应用酶联免疫吸附试验（ELISA）检测梅毒螺旋体抗体具有较好的临床价值，与TPPA比较差异不大，值得推广和应用。 　　[关键词] 酶联免疫吸附试验；明胶颗粒凝集试验；梅毒螺旋体抗体 　　[中图分类号] R446.61[文献标识码] B[文章编号] 1673-9701（2012）10-0084-02 　　Blood transfusion before Treponema pallidum antibodies by ELISA in clinical research 　　CHEN Weiyue1 HU Yanqin2 　　1.Ningbo Central Blood Bank，Ningbo 315040，China；2.Department Laboratory, Longsai Hospital of Zhenhai，Ningbo 315200，China 　　[Abstract] Objective To investigate the blood enzyme-linked immunosorbent assay （ELISA） antibody detection of Treponema pallidum clinical value. Methods Comparative analysis of two tests used to detect Treponema pallidum antibody positive rate and the different age groups measured by ELISA false-positive rates. Results All of 5 790 cases observed object selected，the application of ELISA to detect Treponema pallidum antibody （TP-Ab） positive，50 cases of the positive rate was 0.864%. The application of TPPA detection of Treponema pallidum antibodies in 49 cases，the positive rate was 0.846% （49/5 790）. Miss two detection methods differ significantly (χ2=0.643，P 0.05). ≥70-year-old group，ELISA was used to detect Treponema pallidum antibody positive rate was significantly lower than the 70-year-old group，the difference was statistically significant (P 0.05). Conclusion Transfusion enzyme-linked immunosorbent assay detection of Treponema pallidum antibody with better clinical value，with little difference between the TPPA，worthy of promotion and application. 　　[Key words] Enzyme-linked immunosorbent assay；Gelatin particle agglutination test；Treponema pallidum antibodies 　　目前国内最常用的梅毒的血清学检查主要为TRUST、ELlSA、TPPA等[1]。为了了解输血前人群梅毒的感染情况，本