输血前应用ELISA和TPPA检测梅毒螺旋体抗体效果比较.docVIP

输血前应用ELISA和TPPA检测梅毒螺旋体抗体效果比较 　　[摘要] 目的 比较输血前应用ELISA和TPPA检测梅毒螺旋体抗体的效果。 方法 选择2013年1月～2014年2月在我院接受输血治疗患者3683例的临床资料进行回顾性分析。分别采用ELISA和TPPA法在输血前对血清梅毒螺旋体抗体进行检测。比较两种检测方法的阳性率以及不同年龄组ELISA法检测假阳性率。 结果 3683例患者中，ELISA法检测结果显示33例为梅毒螺旋体抗体阳性，阳性率为0.896%；TPPA检测结果显示32例患者梅毒螺旋体抗体阳性，阳性率为0.869%，两种检测结果阳性率比较差异无统计学意义（P0.05）。≥70岁患者1例为假阳性，假阳性率0.18%；70岁无假阳性结果，两组假阳性检测结果比较，差异有统计学意义（P0.05）。结论 对输血治疗的患者输血前采用ELISA方法进行梅毒螺旋体检测，和TPPA的检测结果差异不大，尤其是对年龄70岁的患者。 　　[关键词] 输血；明胶颗粒凝集试验；酶联免疫吸附试验 　　Comparison between ELISA and TPPA detection for treponema pallidum antibodies before transfusion 　　QIN Jinsong 　　Clinical Laboratory，Changyang Tujia Autonomous County TCM Hospital in Hubei Province， Changyang 443500，China 　　[Abstract] Objective To compare results of ELISA and TPPA detection for Treponema pallidum antibodies before transfusion. Methods Clinical data of 3683 cases from January 2013 to February 2014 received blood transfusion in our hospital were retrospectively analyzed. ELISA and TPPA methods were used to detect serum antibody of Treponema pallidum before transfusion. Positive rates of two detection methods were compared， and false positive rates of different age groups were detected by ELISA. Results 3683 cases of patients， positive results by ELISA showed 33 cases， and the positive rate was 0.896%， positive results by TPPA showed 32 cases， and the positive rate was 0.869%. Positive rates of two detection methods showed no significant difference（P0.05）. ≥70-year-old patients with one false-positive result，false-positive rate was 0.18%，and 70-year-old patients with no false-positive result. False-positive test results of the two groups showed statistical significance （P0.05）. Conclusion For the treatment of patients with transfusion，before blood transfusion using ELISA to detect Treponema pallidum antibodies，test result shows little difference with TPPA， especially for age 70-year-old patients. 　　[Key words] Transfusion； TPPA； ELISA 　　输血是临床上广泛应用的一种治疗方法，是将血液通过静脉输注给患者。输血前需要对患者进行常规传染病检测。目前明胶颗粒凝集试验（TPP