针刺对脑梗死大鼠骨骼肌ATP酶及血清CKLDH活性影响.docVIP

针刺对脑梗死大鼠骨骼肌ATP酶及血清CKLDH活性影响 　　摘要：目的?墓趋兰∷鹕吮曛久傅幕钚愿谋淙胧郑?探讨针刺对脑梗死后骨骼肌运动功能的改善作用，以进一步揭示其作用机制。方法健康雄性SD大鼠100只，体重（192±9）g，随机分为头针组、体针组、头针+体针组，模型组和空白组。每组20只。采用线栓法制作大鼠大脑中动脉局灶性脑梗死模型，并应用Zea-Longa的5级评分标准对造模大鼠进行筛选。每组分别于针刺治疗后即刻、24 h、48 h股动脉取血离心，检测ATP酶、CK、LDH活性。结果①模型组ATP酶活性在0h明显低于空白组（P0.05）。结论针刺可以增加ATP酶活性，降低血清CK及LDH活性，提高骨骼肌有氧酵解，增加肌细胞膜通透性，减轻骨骼肌损伤。 　　关键词：针刺；脑缺血；大鼠；骨骼肌；ATP酶、CK、LDH活性 　　中图分类号：R255.2文献标志码：A文章编号：1007-2349（2017）04-0087-04Effects of Acupuncture on ATPase and Serum CK and LDH Activity 　　in Skeletal Muscle of Rats with Cerebral Infarction 　　ZHANG Yi1， SHI Jing2， FENG Si-feng2， LIU Mei-fang2， CHEN Zhi-hong2， DU Fu-sheng1 　　（1. Yunnan Uinversity of Traditional Chinese Medicine， Kunming 650500， Yunnan； 　　2. Yunnan Traditional Chinese Medicine Hospital， Kunming 650021， Yunnan） 　　【Abstract】Objective： To study the effect of acupuncture on skeletal muscle motor function after cerebral infarction， and to reveal its mechanism from the changes of skeletal muscle injury marker enzyme activity. Methods： 100 healthy male SD rats weighing 192±9 g were randomly divided into a scalp acupuncture group， a body acupuncture group， a scalp + body acupuncture group， a model group and a blank group， 20 rats per group. The suture method was used to establish rat models of focal cerebral infarction and Zea-Longas five-level scoring standard was used to screen the model rats. The activity of ATPase， CK and LDH of each group were measured by centrifugation at 24 h and 48 h after acupuncture treatment. Results： 1. The activity of ATPase in the model group was significantly lower than that in the blank group （P0.05）. Conclusion： Acupuncture can increase the activity of ATPase， decrease the activity of serum CK and LDH， improve the aerobic glycolysis of skeletal muscle， increase the permeability of muscle membrane and reduce skeletal muscle injury. 　　【Key words】acupuncture， cerebral ischemia， rat， skeletal muscle. ATPase， CK， activity of LDH 　　?X梗死具有极高的致残率，超过2/3的脑梗死患者伴有肢体功能障碍[1～2]，主要包括骨骼肌无力、关节挛缩