钩藤中乌索酸和钩藤碱对肝癌细胞株HepG2影响.docVIP

钩藤中乌索酸和钩藤碱对肝癌细胞株HepG2影响 　　摘要：目的 观察钩藤中乌索酸和钩藤碱对人肝癌细胞株HepG2的影响，探讨其抗肝癌的机制。方法 将50、25、12.5、6.25 μmol/L乌索酸、钩藤碱分别与人肝癌细胞株HepG2共同培养24、48、72 h后，以1%DMSO为阴性对照组，CCK-8法检测HepG2细胞增殖，吖啶橙荧光染色观察50 μmol/L乌索酸、钩藤碱和1%DMSO作用48 h后HepG2细胞形态。结果 乌索酸对HepG2细胞增殖有明显的抑制作用，并且其抑制率与作用时间、药物浓度呈正相关，钩藤碱对HepG2细胞增殖抑制作用较弱。病理观察结果显示，钩藤碱组大部分细胞出现细胞皱缩、包浆致密、核染色边集等凋亡早期改变，而乌索酸组大部分细胞出现核裂解、凋亡小体等凋亡晚期改?。结论 钩藤中乌索酸和钩藤碱对人肝癌细胞株HepG2增殖有抑制作用，能诱导HepG2细胞凋亡。 　　关键词：乌索酸；钩藤碱；HepG2细胞 　　DOI：10.3969/j.issn.1005-5304.2017.05.015 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2017）05-0063-04 　　Effects of Ursolic Acid and Rhynchophylline in Uncariae Ramulus Cum Uncis on Human Hepatoma HepG2 Cells WU Ji-chao1， ZHU-Jie1， NIE Dong-lei1， PAN Si-ying1， HUANG Qun2 （1. Graduate School of Youjiang Medical University for Nationalities， Baise 533000， China； 2. Affiliated Hospital of Youjiang Medical University for Nationalities， Baise 533000， China） 　　Abstract： Objective To investigate the effects of ursolic acid and rhynchophylline in Uncariae Ramulus Cum Uncis on human hepatoma HepG2 cells； To discuss its antihepatoma mechanism. Methods Culture the human hepatoma HepG2 cells with 50， 25， 12.5， 6.25 μmol/L ursolic acid， rhynchophylline for 24， 48， 72 hours respectively. 1%DMSO was set as negative control group. CCK-8 based cytotoxicity assay was used to detect the growth of HepG2. Acridine orange fluorescent staining was used to observe human hepatoma HepG2 cells cultured with 50 μmol/L ursolic acid， rhynchophylline and 1%DMSO for 48 hours were observed. Results Ursolic acid significantly acted as a disincentive to the growth of HepG2 cells， which was in positive correlation with time and concentration. Inhibited effect by rhynchophylline on HepG2 cell proliferation was weaker than ursolic acid. Pathological observation showed that most of the cells in rhynchophylline group showed cell shrinkage， dense pulp coating and nuclear staining edge set， and most of the cells in the ursolic acid group showed apoptosis late changes， such as nuclear cleavage a