阿托伐他汀对对比剂肾病大鼠肾脏细胞凋亡及FASFASL表达影响.docVIP

阿托伐他汀对对比剂肾病大鼠肾脏细胞凋亡及FASFASL表达影响 　　[摘要] 目的 研究阿托伐他汀对对比剂肾病大鼠肾脏细胞凋亡及FAS/FASL表达的影响。 方法 清洁级雄性SD大鼠32只随机分为A（正常组）、B（实验对照组）、C（造模组）、D（他汀组）4组，每组8只。他汀组于造模前3 d至造模后3 d给予阿托伐他汀30 mg/（kg·d）灌胃，其余组给予等体积的生理盐水灌胃。建立对比剂肾病大鼠模型，其中C、D组注射吲哚美辛、N—硝基—L—精氨酸甲酯、泛影葡胺，B组以生理盐水代替泛影葡胺，A组只注射等体积的生理盐水。采集各组大鼠造模前、造模后48 h的血液，用全自动生化分析仪检测血肌酐（SCr）值；造模72 h后处死大鼠摘取右肾，采用原位末端标记法（TUNEL）检测肾脏细胞凋亡情况；免疫组化法检测肾脏FAS、FASL表达变化。 结果 ①C组造模前血SCr较造模后升高25%（P 0.05），且造模后C组血SCr较A、B、D各组有所升高（P 0.05）；②C组较A、B组肾脏凋亡细胞数明显增多，FAS和FASL的表达亦显著增强（P 0.05），D组较C组凋亡细胞数减少，FAS和FASL的表达减弱（P 0.05）。 结论 阿托伐他汀可能通过减弱凋亡相关蛋白FAS和FASL的表达而抑制肾脏细胞凋亡，从而发挥其预防保护作用。 　　[关键词] 阿托伐他汀；对比剂肾病；细胞凋亡；大鼠；FAS/FASL 　　[中图分类号] R692.9 [文献标识码] A [文章编号] 1673—9701（2012）24—0020—03 　　The effects of atorvastatin on apoptosis and the expression of FAS and FASL in the kidney of contrast—induced nephropathy in rats 　　LIU Qing DAI Chun 　　Department of Nephrology，the Affiliated Hospital of Xuzhou Medical College，Xuzhou 221000，China 　　[Abstract] Objective To investigate the effects of atorvastatin on apoptosis and the expression of FAS and FASL in the kidney of contrast—induced nephropathy in rats. Methods Thirty—two clean male SD rats with mean of weight （200±10） g were randomly divided into four groups，8 rats in each group. There were A group（normal group），B group （experimental control group），C group （contrast—induced nephropathy group），D group （atorvastatin intervention group）. In D group， atorvastatin was gavaged from 3 before days modeling to 3 days after it， and the remaining group was given an equal volume of saline. Establish the rat model of the contrast—induced nephropathy. Indomethacin，N—nitro—L—arginine methyl ester and diatrizoate were all injected in C and D groups，when the group B with aline instead of diatrizoate， group A only injected with saline. Then collected the blood before modeling and 48h after modeling. Serum creatinine （SCr） were detected by automatic biochemistry analyzer. After modeling 72 h， rats were killed to get the right kidney. Apoptosis was evaluated by means