香兰素对人精子活力影响.docVIP

香兰素对人精子活力影响 　　摘要：目的：香兰素在日常生活中用途非常广，如在日化、食品，烟草工业，临床药物的制造等。目前国内还没有相关报道香兰素对人生殖系统有影响。本实验探讨香兰素对男性精子活力的影响。方法： 将香兰素分成不同的浓度组：1X10-2MMOL/ML ，1X10-3MMOL/ML ，1X10-4MMOL/ML， 1X10-5MMOL/ML， 1X10-6MMOL/ML，1X10-8MMOL/ML加入符合WHO第四版规定的正常的人精液中。利用计算机辅助精子分析仪，检测与比较对精子的活力影响。结果：随着香兰素浓度的逐减对精子活力的影响越来越小，浓度为1X10-6MMOL/ML，精子活力变化最小，与生理盐水，灭菌水比较无显著差异，通过数学统计分析无统计学意义。结论： 香兰素是一种用途广泛相对安全的食品添加剂，在国家规定标准范围内使用对人精子活力无影响。对人类的其他影响还有待探讨。 　　关键词：香兰素 食品添加剂 人精子活力 　　中图分类号 ：R715.5 文献标识码：A 　　ABSTRACT：Objective： Vanillin is used very widely. For example it is used in daily chemical production， food， the tobacco industry and manufacture of clinical medicine. So far， there are no relevant reports about the affect of vanillin on human reproductive system in China. This test is to discuss the influences of vanillin on the male sperm vitality. Methods： The concentration of vanillin is divided into different groups： 1X10-3MMOL/ML ，1X10-4MMOL/ML， 1X10-5MMOL/ML， 1X10-6MMOL/ML，1X10-8MMOL/ML. And then they were added to the normal semen. By computer assisted sperm analyzer， testing and comparison their influence on sperm vitality. Results： As the vanillin concentration decreases， its effect is more and more smaller on sperm motility. The change is minimal on the concentration of 1X10-6MMOL/ML. And there are no significant differences compared with normal saline and water sterilization. Through mathematical statistical analysis， there is no statistical significance. Conclusions： Vanillin is a kind of relative safety and used widely food additive. There is no influence sperm motility if it was used within the scope of the provisions of the state standard. But the other more effects on human remains to be explored. 　　KeyWords ：Vanillin food additives human sperm motility 　　香兰素的用途非常广泛，可以作为香原料，定香剂或矫味剂应用于食品，是一种常用的食品添加剂，常用于饮料，糕点，糖果，饼干，炒货和面包中【1-3】；用于工业，作锌电镀的活性剂、镀锌槽的增白剂、亚麻子油的氧化助剂及杀虫剂的引诱剂【4】；还可以用于制烟中防止吸烟引起的嘴唇干燥【5】；另，香兰素可以作为一种催熟剂、维生素B2的增溶剂和甲基异丁烯聚合的催化剂。香兰素也是重要的化学中间体，用作药物中生产L_多巴和L-甲基多巴、罂粟碱的基础原料。还是抗肿瘤药吉非替尼的中间体，以异香兰素为原料合成【6】乙基香兰素、香草酸乙