香根草对重金属镉积累及耐性研究.docVIP

香根草对重金属镉积累及耐性研究 　　摘要: 通过香根草的水溶液培养,研究了不同浓度的镉对香根草的生理和生态的影响.结果表明:香根草富集的镉主要被累积在根内,最高富集系数达到24.06%.溶液Cd浓度与香根草根内Cd的累积量显著正相关(r=0.9874),与根的耐性指数呈显著负相关(r=-0.9436).低浓度Cd(≤1 mmol#8226;L-1)处理使根系活力和硝酸还原酶(NR)活力随时间呈现先增后降的趋势,中、高浓度Cd (≥1.5 mmol#8226;L-1)处理使根系活力呈显著下降的趋势,且浓度越高,下降幅度越大.化学形态分析表明,在香根草体内Cd的NaCl提取态占绝对优势,在根和茎叶内的平均占52.06%和64.03%.可以认为香根草在镉胁迫下其氮代谢,呼吸作用,植物的生长发育受到一定程度抑制,但其对镉仍具有较强的耐性和富集能力. 　　关键词: 香根草;镉;富集系数;根系活力;硝酸还原酶 　　中图分类号: X53 文献标识码:A文章编号: 　　Study of the Cadmium Accumulation and Tolerance of Vetiveria zizanioides 　　LIU Yun guo, SONG Xiao-chen, WANG Xin,MIN Zong-yi, Liu Si mian 　　(College of Environmental Science and Engineering, Hunan University, Changsha 410082, China) 　　Abstract: The physiological and ecological effects on Vetiveria zizanioides(V.zizanioides) grown in hydroponic culture under the stresses at different concentration of Cadmium(Cd)were studied.The experimental results show that Cd mainly cumulated in root.The highest Bioconentration factor(BF) is 34%. of Cd had a apositive correlation with Cd cumulation in root (r=0.9874),while the concentration has negative correlation with tolerace index of root(r=-0.94362). the root activity and nitrate reductase(NR)activity appeared an increase and then a decrease trend in low concentration treatment of Cd (≤1mmol#8226;L-1)with time;the root activity and nitrate reductase activity appeared an obvious descending trend in middle and high concentration treatment of Cd (≥1.5mmol#8226;L-1,and descending extent increased with concentration of Cd increase. Sequential extraction revealed thatNaCl extractable-Cd was dominant in both roots and leaves of V.zizanioides.The average proportion is 52.06 and 64.03. V.zizanioides nitrogen metabolism ,respiration and growth development inhibited by Cd stress,it still has strong tolerance level and accumulation ability. 　　key words: Vetiveria zizanioidesCadmiumBioconentration factor(BF)root activitynitrate reductase 　　 　　重金属污染对生物有机体能产生严重的影响,其在食物链中的生物富集对人类和生态环境极具危险性1.近年来,随着镉矿床的开采以及有色