香加皮有效成分提取工艺和检测方法研究进展.docVIP

香加皮有效成分提取工艺和检测方法研究进展 　　[摘要] 香加皮是用于治疗类风湿性关节炎的传统中药材。随着现代研究的不断深入，其相关药理作用的起效成分逐步明确，相应的制备工艺和检测手段也逐渐完善。本文简述了香加皮中五大类有效成分的提取工艺和检测方法：C21甾体苷类一般采取乙醇溶液提取，使用大孔树脂柱纯化或者制备液相进行分离制备，多以HPLC-UV法（高效液相色谱法）进行分离检测；强心苷类用甲醇或乙醇超声提取，采用紫外分光光度法和HPLC法检测；醛类采用硅胶、十八烷基硫酸钠键合相（ODS）柱色谱等方法处理，HPLC法和气相色谱-质谱联用法（GC-MS法）分析；脂肪酸类和其他类的提取和检测方法也有涉及，为香加皮中相关成分的新药研发提供参考。 　　[关键词] 香加皮；有效成分；提取工艺；检测方法；C21甾体苷；强心苷 　　[中图分类号] R927.11 [文献标识码] A [文章编号] 1673-7210（2016）06（c）-0037-05 　　[Abstract] Cortex Periplocae is one kind of traditional Chinese medicines for the treatment of rheumatoid arthritis. With the continuous research， the related pharmacological ingredients have been discovered gradually while the corresponding extraction process and analytical method have been widely studied. The extraction process and analytical method of 5 kinds of ingredients in cortex periplocae were summrized in this paper. C21 steroidal glycosides were generally extracted by ethanol， purified by macroporous resin or separated by preparative HPLC method， detected by ultraviolet-visible detect. Cardiac glycosides were ultrasonic extracted with methanol or ethanol. Cardiac glycosides were quantitatively analyzed by ultraviolet-visible spectrometry or HPLC method. Aldehydes were purified by silica gel and ODS column chromatography， and analyzed by HPLC or GC-MS. This paper also summrized fatty acids and other ingredients. The advances in research on the extraction process and analytical method have been reviewed in this paper， in order to provide references for development of the related new drugs. 　　[Key words] Cortex Periplocae； Effeective ingredients； Extration process； Analytical method； C21 steroidal glycosides； Cardiac glycosides 　　香加皮Cortex Periplocae为萝摩科植物杠柳Periploca sepium Bunge 的根皮，又被称为北五加皮、杠柳皮或者香五加皮。最早记载在古籍《神农本草经》[1]，《中国药典》自1977年也均有收载，功能主治为祛风湿、强筋骨，用于心悸气短、下肢浮肿、风寒湿痹、腰膝酸软。 　　香加皮中药效活性成分主要分为C21甾体苷、强心苷、醛类化合物和有机酸类、三萜类、寡糖等化合物[2-5]，其中C21甾体苷和强心苷的药理活性被作为主要研究对象[6]，在2015年版《中国药典》中醛类化合物4-甲氧基水杨醛为中药材的含量控制指标[7]。由于不同类型化合物的极性相差较大，根据目标成分不同选择的提取工艺差异也较