鼠曲草花中黄酮提取及抗氧化活性研究.docVIP

鼠曲草花中黄酮提取及抗氧化活性研究 　　摘要 鼠曲草是一年生草本植物，在我国均有分布，民间清明前后采食。本文检测了鼠曲草茎叶及花中总黄酮含量，对比了提取方法。总黄酮用醋酸铅沉淀、柱层析纯化。结果表明，鼠曲草茎叶及花中的黄酮含量分别为7.37、38.14 mg/g；最佳提取条件为乙醇浓度40%、料液比1∶40，温度70 ℃，时间2 h；黄酮（A）的含量最多，还原能力和清除DPPH自由基能力高于芦丁，有明显的抗猪油氧化能力，但抗氧化持续性不及BHT。黄酮（B）的抗氧化活性与芦丁相当。 　　关键词 鼠曲草；花；提取；总黄酮；抗氧化活性；过氧化值 　　中图分类号 TS201.2 文献标识码 A 文章编号 1007-5739（2017）24-0237-04 　　Study on Extraction and Antioxidant Activity of Flavonoids from Flower of Gnaphalium affine D. Don 　　ZHANG De-sheng KANG Zhao-jin YOU Shuang-mei XIA Wan-qiu FENG Ya-juan LIU Pin-hua 　　（College of Chemistry and Environmental Science，Qujing Normal University，Qujing Yunnan 655011） 　　Abstract Gnaphalium affine D.Don is an annual herb，which distributes widely in China and is often fed in Tomb-Sweeping Day.This paper tested the content of total flavonoids in stem，leaves and flowers of Gnaphalium affine D.Don，and compared the extraction methods.Total flavonoids were precipitated with lead acetate and purified by column chromatography.The results showed that the content of flavonoids in the stem and leaves，flowers of Gnaphalium affine D.Don were 7.37 mg/g and 38.14 mg/g，respectively.The optimum extraction conditions were as following，ethanol concentration was 40%，solid-liquid ratio was 1∶40，temperature was 70 ℃，time was 2 h.The content of flavonoids（A）was highest，reducing ability and DPPH free radical scavenging ability were higher than that of rutin，which had obvious anti-lard oxidation ability，but the persistence of antioxidation was not as good as BHT. Flavonoids（B）had similar antioxidant activity to rutin. 　　Key words Gnaphalium affine D.Don；flower；extraction；total flavonoid；antioxidant activity；peroxidation value 　　鼠曲草（Gnaphalium affine D.Don），菊科，簇生，一年生草本，生于海拔1 600～2 700 m的田埂、荒地、路旁，尤以稻田最常?。清明前民间有采集嫩尖食用的习惯。茎叶入药，化痰止咳、清热利湿，活血降压，是治气喘和支气管炎以及非传染性溃疡、创伤的常见用药，全草含胡萝卜素、抗坏血酸、硫胺素、核黄素、花中木犀草素4′-β-D-葡萄糖苷、鼠曲草素、斛皮素-5-甲醚，有镇咳、抑菌的作用[1-2]。现有研究表明，鼠曲草属植物具有多种生物活性，除了医药价值，在营养保健、生物农药、化妆品等方面也有一定的应用价值。鼠曲草资源丰富，药食两用，是一种极具开发利用潜质的生物资源[3]。因此，本文研究了鼠曲草花中黄酮的提取方法及抗氧化活性，以期为深