东亚飞蝗内质网I型信号肽酶亚基(LmSPC1)的基因克隆与功能分析-生物学专业论文.docxVIP

ABSTRACT The delivery of secreted proteins to their proper destination depends on a membrane-bound signal peptidase. In single-cell organisms, the type I signal peptidase gene is vital for cell viability. However, knowledge of the functions of this gene in multicellular organisms is still limited. The migratory locust (Locusta migratoria) is a typical hemimetabolous insect and an excellent model system for the study of development, neuroscience, physiology and evolution. Moreover, it is one of the most destructive pests and causes great losses in crops every year on a global scale. Nowadays, most of the pest control methods based on traditional chemical pesticides, especaily organophosphate (OP) insecticides. Undoubtedly, these kinds of control measures casued a series of ecological, environment and safty problems mainly including pest resistance, pesticides residue and pest resurgence. Therefore, a new pest control strategy based on low poison, high efficiency and safety, is urgent to be developed to solve these problems. Fortunately, RNAi technology provided a possibility measure for this orientation. However, the successful performation of this new strategy relies on a premise that developed adequate efficient and essential target genes originating from pest itself. Consequently, looking for and research the locust essential gene that affected their growth and development has important theory significance and practice application significance so as to provide potential novel target for pest control. The main results are as follows: ① Gene clone and sequence analysis of LmSPC1 A type I signal peptidase subunit (LmSPC1), was cloned from Locusta migratoria manilensis，accession number KC576515. LmSPC1 cDNA had a 537 bp ORF that encoded 178 amino acids with a predicted molecular weight of 21 kDa, and contained all five highly conserved structures of type I signal peptidases. ② Expression profile of LmSPC1 in different tissues A semi-quantitative RT-PCR was performed