甘肃黄芪与红芪总黄酮含量对比测定方法探索研究.docVIP

甘肃黄芪与红芪总黄酮含量对比测定方法探索研究 　　摘要：目的 探索建立甘肃黄芪和红芪药材中总黄酮含量对比的测定方法。方法 以毛蕊异黄酮苷等为对照品，采用紫外分光光度法和比色法（显色剂：NaNO2-Al（NO3）3-NaOH、AlCl3、Mg（Ac）2、NaOH、磷钼酸、盐酸-镁粉），考察样品和对照品吸收曲线。结果 采用比色法，黄芪、红芪样品和对照品的最大吸收波长不一致，峰形有差别或差别较大。采用紫外法测定，毛蕊异黄酮苷在Ⅱ带260 nm处呈现特征肩峰吸收，黄芪和红芪亦在Ⅱ带呈现特征肩峰吸收，吸收波长分别为263、265 nm，吸收波长与对照品基本一致。结论 常用的显色反应不适合甘肃黄芪和红芪中总黄酮含量的对比测定；异黄酮类化合物吸收Ⅱ带作为特征吸收带，适于黄芪和红芪样品中总黄酮含量的对比研究。 　　关键词：黄芪；红芪；总黄酮；紫外-可见分光光度法；比色法；含量测定 　　DOI：10.3969/j.issn.1005-5304.2016.09.024 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2016）09-0099-07 　　Study on Determination Method of Total Flavonoids in Gansu Astragali Radix and Hedysarum Polybotrys YE Ying1， BAO Qiang2， WANG Rui-hai1， BAI Dong1， XUE Xin1， ZHANG Li-shi1， LIU Li-mei1 （1. Institute of Basic Theory Research of TCM， China Academy of Chinese Medical Sciences， Beijing 100700， China； 2. Department of Pharmacy， Gansu Province Hospital of TCM， Lanzhou 730050， China） 　　Abstract： Objective To study the determination method of total flavonoids in Gansu Astragali Radix and Hedysarum Polybotrys. Methods Calycosin glycosides etc. was selected as reference substances， comparison on the difference of absorption curves was done by ultraviolet spectroscopy and colorimetric method （NaNO2-Al（NO3）3- NaOH， AlCl3， Mg（Ac）2， NaOH， phosphomolybdic acid， HCl-Mg power）. Results With colorimetric method， the maximum absorption wavelength of referrence and the test was inconsistent. The absorption peak shape was also different. With UV method， Calycosin glycosides in band Ⅱ （260 nm） showed a shoulder absorption. Astragali Radix and Hedysarum Polybotrys also showed characteristic shoulder absorptions in band Ⅱ with absorption wavelength at 263 nm and 265 nm. So the sample absorption wavelength is basically the same as that of the control sample. Conclusion Colorimetries usually used for determination of total flavonoids are not suitable for the comparison determination of Gansu Astragali Radix and Hedysarum Polybotrys. It is suitable for determining the contents of total flavonoids in samples