化橘红药材挥发油提取及用饱与水溶液法包合工艺研究.docVIP

化橘红药材挥发油提取及用饱与水溶液法包合工艺研究 　　摘要：目的 优选化橘红药材挥发油提取及包合的最佳工艺。方法 以挥发油得率为指标，采用单因素试验考察挥发油的提取工艺；以挥发油包合率及包合物得率为评价指标，采用饱和水溶液法，进行L9（34）正交试验，优选挥发油的包合工艺，并采用显微呈像分析、X射线衍射法对包合物进行表征。结果 挥发油的最佳提取工艺为不浸泡，加10倍量水，提取10 h；最佳包合工艺为：油（mL）∶β-环糊精（g）∶水（mL）=1∶8∶80，包合温度为50 ℃，包合时间为3 h。包合工艺的验证试验显示，挥发油包合率、包合物得率平均值分别为91.50%、88.36%。显微镜法、X射线衍射法表明包合物形成，包合物状态良好。结论 本试验优选出的挥发油提取及包合工艺稳定可行，可为制剂生产提供一定依据。 　　关键词：化橘红；挥发油；提取；β-环糊精；包合工艺 　　DOI：10.3969/j.issn.1005-5304.2017.11.016 　　中图分类号：R284.2 文献标识码：A 文章编号：1005-5304（2017）11-0067-04 　　Study on Extraction and Inclusion Process of Volatile Oil from Citri Grandis Exocarpium with Saturated Aqueous Solution WANG Wen-ping， YIN Xing-bin， DONG Xiao-xu， YANG Chun-jing， ZHANG Hai-ying， YOU Long-tai， NI Jian （College of Pharmacy， Beijing University of Chinese Medicine， Beijing 100102， China） 　　Abstract： Objective To optimize the processes of extraction and inclusion of volatile oil from Citri Grandis Exocarpium. Methods With yield ratio of volatile oil as evaluation index， single factor experiments were used to study the extraction process of volatile oil. With the inclusion rate of the volatile oil and the yield of inclusion as evaluation indexes， saturated aqueous solution was used to the L9（34） orthogonal experiments to reach the best inclusion process optimization. And the microscopic imaging analysis and X-ray scattering technology were adopted to character the inclusion compound. Results The optimum extraction process of volatile oil was extracted for 10 hours with 10 folds the amount of water， and without soaking. The optimum conditions of inclusion process were as follows： volatile oil （mL）： β-CD （g）： water （mL） ratio was 1：8：80； inclusion temperature was 50 ℃； inclusion time was 3 hour. The validation experiments of the inclusion rate of the volatile oil and the yield of inclusion were 91.50% and 88.36%. Microscopic imaging analysis and X-ray scattering technology proved the inclusion compound had been formed. Conclusion Optimal extraction and inclusion process are