GSTP1、GSTM1基因多态性与顺铂导致骨髓抑制的相关性研究.PDFVIP

现代药物与临床 Drugs Clinic 第 33 卷 第 9 期 2018 年 9 月 ·2165 · GSTP1、GSTM1 基因多态性与顺铂导致骨髓抑制的相关性研究 郭书英，吴传亮，孙 慧，刘 卫* 解放军第 88 医院，山东 泰安 271000 摘 要：目的 考察顺铂致骨髓抑制与谷胱甘肽硫转移酶 P1 （GSTP1）和谷胱甘肽硫转移酶M1 （GSTM1）基因多态性的关 系。方法 用病例对照研究的方法考察以顺铂为主要化疗方案的 100 例癌症初治肿瘤化疗患者基因多态性与骨髓移植的相关 性，白细胞计数和血小板的数量为指标判断是否发生了骨髓抑制以及骨髓抑制程度。提取 DNA 扩增测序法判断 GSTP1、 GSTM1 基因型。结果 GSTP1 的 3 种基因型 AA 、AG 、GG 型均存在，并且在 62 例发生骨髓抑制的病例中，GSTP1 AG/GA 型的患者的分布明显高于 GSTP1 AA 型患者。GSTM1 包含缺失（－）和非缺失（＋）2 种基因型，100 例病例中 GSTM1 （＋） 少于 GSTM1 （－）。Logistic 分析结果表明 GSTP1 AG/GG 型是顺铂致骨髓抑制的独立危险因素，GSTM 基因缺失虽不是顺铂 致骨髓抑制的独立危险因素，但能促进 GSTP1 AG/GG 型的作用，导致顺铂致骨髓抑制进一步加重。结论 顺铂导致的骨髓 抑制程度与 GSTP1 基因多态性相关。 关键词：顺铂；骨髓抑制；GSTP1；GSTM1；基因多态性 中图分类号：R965 文献标志码：A 文章编号：1674 - 5515(2018)09 - 2165 - 04 DOI: 10.7501/j.issn.1674-5515.2018.09.002 Relationship between bone marrow suppression induced by cisplatin and GSTP1 or GSTM1 gene polymorphism GUO Shu-ying, WU Chuan-liang, SUN Hui, LIU Wei The 88th Hospital of PLA, Taian 271000, China Abstract: Objective To investigate the relationship between bone marrow suppression induced by cisplatin and GSTP1 or GSTM1 gene polymorphism，Methods Case-control study was adopted to investigate 100 newly diagnosed cancer patients undergoing chemotherapy treated with cisplatin as chemotherapy regimen. WBC and PLT were determined as marrow suppression index. GSTP1 and GSTM1 genotypes were determined by PCR method. Results GSTP1 had three genotypes as AA, AG, and GG gentype. Among 62 cases suffering from marrow suppression, distributions of GSTP1 genotype AG/GA were higher significantly than those of GSTP1 AA gentype. GSTM1 included 2 genotypes as deletion ( －) and non deletion ( ＋), and cases of GSTM1 ( ＋) were less than GSTM1 ( －). Logistic analysis showed that GSTP1 AG/GG type was an independent risk factor for cisplatin-induced mye