一株DDT降解菌的筛选及其降解特性.PDFVIP

中国环境科学 2018,38(5) ：1935~1942 China Environmental Science 一株 DDT 降解菌的筛选及其降解特性 * 冯玉雪,毛 缜 , 吕蒙蒙 ( 中国矿业大学(徐州)环境与测绘学院,江苏 徐州 221116) 摘要： 以 DDT 为目标污染物,通过筛选获得了一株效果稳定的 DDT 降解菌,并对其进行形态学观察,生理生化特性及 16S rRNA 测序鉴定. 经鉴定,该菌株属于甲基菌属(Methylovorus),命名为 Methylovorus sp. XLL03.菌株在 pH 值为 7,温度 30°C,外加碳源浓度 0.5%,初始 DDT 浓 度 20mg/L 时生长量最大.在 pH 值为 6,温度 30℃下,外加碳源(葡萄糖)浓度 0.1%,初始 DDT 浓度 20mg/L 时对 DDT 的降解率最大.在优化条 件下,4d 后菌株 XLL03 对 DDT 最高降解率可达 50.4%.利用 GC-MS 对 DDT 的降解中间产物进行定性分析,初步推断在菌株 XLL03 中,DDT 最初分别通过脱氯和脱氯化氢生成 DDD 和 DDE, 随后 DDD 和 DDE 进一步脱氯得到 DDMU,最终 DDMU 开环后又经过一系列反应被彻 底矿化.在 DDT 的代谢过程中,未发现代谢中间产物的积累,表明菌株 XLL03 在修复受 DDT 污染的水或土壤中具有一定的应用前景. 关键词：DDT ；微生物降解；降解特性；降解途径 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2018)05-1935-08 * Screening and degradation characteristics of a DDT-degrading bacteria. FENG Yu-xue, MAO Zhen , LÜ Meng- meng (School of Environmental Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, China). China Environmental Science, 2018,38(5)：1935~1942 Abstract ：DDT was used as the target pollutant, and a strain capable of degrading DDT was obtained by isolation and the identification of the strain was carried out through morphological observation, experiments on physiological and biochemical characteristics and 16S rRNA sequencing. The strain was identified as Methylovorus, and named as Methylovorus sp. XLL03. The best growing condition for the strain was as follow: pH of 7, 30°C, external carbon source concentration of 0.5% , and initial DDT concentration of 20mg/L. The best degradation condition for the strain was as follow: pH of 6, 30°C, external carbon source(glucose) concentration of 0.1%, and initial DDT concentration of 20mg/L. Under t