百色市某医院白假丝酵母菌基因分型和耐药相关性分析.docVIP

百色市某医院白假丝酵母菌基因分型和耐药相关性分析 　　【摘要】目的分析当地常见的白假丝酵母菌基因型及其耐药性。方法收集2011年1月～2013年1月右江民族医学院附属医院190株白假丝酵母菌菌株，应用PCR技术对其25SrDNA中的transposable intron基因进行基因型分析。应用MicroScan全自动细菌鉴定仪，对标本分离出的白假丝酵母菌进行鉴定和药物敏感试验。结果感染的190株白假丝酵母菌大多数为A型，共112株（58.95%）；A型对5氟胞嘧啶、伊曲康唑和伏立康唑的耐药率高于B型和C型；A、B、C三型对氟康唑全部耐药，而对两性霉素均敏感。结论不同基因型的白假丝酵母菌对药物的敏感性不同，应针对不同基因型别的白假丝酵母菌应用不同的药物进行治疗。 　　【关键词】白假丝酵母菌；基因分型；耐药性 　　中图分类号：R34 文献标识码：ADOI：10.3969/j.issn2015.03.003 　　Analysis of correlation of genotyping and drug resistance 　　of candida albicans in a hospital in Baise 　　CHEN Wencheng，NONG Legen，QIN Yuhua▲ 　　（Affiliated Hospital of Youjiang Medical University for Nationalities，Baise 533000，Guangxi，China） 　　【Abstract】ObjectiveTo analyze genotyping and drug resistance of candida albicans which is normally seen in the local region.MethodsA total of 190 strains of candida albicans were collected from Affiliated Hospital of Youjiang Medical University for Nationalities from January，2011 to January，2013.Transposable intron gene in 25SrDNA were analyzed by polymerase chain reaction （PCR），identification of candida albicans which was isolated from the specimen and its drug sensitivity test were performed by MicroScan automatic bacterial identification instrument.Results Majority of the 190 infected candida albicans were type A，with a total number of 112 strains（58.95%）.Drug resistance rate of genotype A to 5fluorocytosine，itraconazole and voriconazole was significantly higher than those of genotype B and genotype C.Genotype A，genotype B and genotype C were all resistant to fluconazole，but sensitive to amphotericin.ConclusionDifferent genotypes of candida albicans has different sensitivity to drugs.Thus，different genotypes of candida albicans should be treated by different drugs. 　　【Key words】candida albicans；genotype；drug resistance 　　白假丝酵母菌（Candida albicans）是人体内常见的条件致病真菌，近年来，由于滥用广谱抗生素、大量使用免疫抑制剂以及广泛运用侵入性技术等原因，导致白假丝酵母菌的感染率显著增加。白假丝酵母菌感染的特征之一就是存在地区差异，不同地区引起感染的白假丝酵母菌的类型不一样[1]，因此，研究不同地区白假丝酵母菌的基因型和耐药性有十分重要的意义。本研究应用PCR技术和常规