白细胞介素―23RArg381Gln基因多态性和炎症性肠病相关性研究.docVIP

白细胞介素―23RArg381Gln基因多态性和炎症性肠病相关性研究 　　【摘要】 目的：探讨白细胞介素-23R Arg381Gln基因多态性与炎症性肠病（Inflammatory Bowel Disease，IBD）相关性。方法：采用序列特异性引物配合聚合酶链反应（polymerasechain reaction with sequence specific primers，PCR-SSP）技术对125例炎症性肠病患者（研究组：UC组78例，CD组47例）与120例健康体检者（对照组）IL-23R基因非同义单核苷酸多态性（IL-23R Arg381Gln）进行分析，计算等位基因的表现频率，评估IL-23R Arg381Gln基因多态性与炎症性肠病相关性。结果：UC组、CD组、对照组A等位基因的表现频率分别为5.13%、2.13%、5.83%，三者比较差异无统计学意义（P0.05），但CD组A等位基因的表现频率稍低于UC组与对照组。UC组、CD组、对照组G等位基因的表现频率分别为6.41%、6.38%、5.83%，比较差异无统计学意义（P0.05）。结论：白细胞介素-23R Arg381Gln基因多态性与炎症性肠病无显著的关系，个体遗传差异性可能决定炎症性肠病的易感性。 　　【关键词】 白细胞介素-23R； Arg381Gln； 基因多态性； 炎症性肠病 　　【Abstract】 Objective：To study the correlation between interleukin-23R Arg381Gln gene polymorphism and inflammatory bowel disease.Method：By sequence specific primers and PCR technique in 125 cases of inflammatory bowel disease（study team：UC group of 78 cases，CD group of 47 cases）and 120 healthy check-up （control group）IL-23R genes non-synonymous single-nucleotide polymorphisms were analyzed and the allele performance frequency were calculated.The correlation between IL-23R Arg381Gln gene polymorphism and inflammatory bowel disease was evaluated.Result：The allele performance frequency of UC group，CD group and control group were 5.13%，2.13%，5.83%，the comparison difference among groups had no statistical significance （P0.05），but A allele performance frequency of CD group was slightly lower than UC group and control group.The G allele performance frequency of UC group，CD group and control group were 6.41%，6.38%，5.83%，compared among groups with no significant difference（P0.05）.Conclusion：It has no significant correlation between IL-23R Arg381Gln gene polymorphism and inflammatory bowel disease，the individual genetic differences may determine the susceptibility of inflammatory bowel disease. 　　【Key words】 Interleukin-23R； Arg381Gln； Genetic polymorphism； Inflammatory bowel disease 　　First-author’s address：Shenzhen Baoan District Central Hospital，Shenzhen 518102，China 　　doi：10.3969/j.issn.