两种土荆皮习用药材提取物体外抗真菌活性的研究.docVIP

两种土荆皮习用药材提取物体外抗真菌活性的研究 　　【摘要】 目的 研究市场两种习用土荆皮代用药材的体外抗真菌活性，为土荆皮的习用药材(水翁皮与余甘子皮)的药理应用提供依据。方法 采用药基琼脂稀释法，研究水翁皮与余甘子皮的提取物对11株浅表性皮肤癣菌的最小抑菌浓度(MIC)和最低杀菌浓度(MFC)。结果 土荆皮的提取物对武汉须毛癣菌、武汉近平滑癣菌的MIC和MFC 3.13 μl/ml,对其余9株皮肤癣菌的MIC和MFC 6.25 μl/ml,水翁皮与余甘子皮的提取物对试验11株皮肤癣菌的MIC和MFC50 μl/ml, 达克宁的MIC和MFC0.78 μl/ml。结论 土荆皮的两种习用药材水翁皮与余甘子皮体外对11株皮肤癣菌未显示出抗真菌活性。? 　　【关键词】 　　土荆皮；水翁皮；余甘子皮；抗真菌活性；体外 　　?? 　　Study on antifungal activity of cortex pseudolarici?s alternative medicine in vitro 　　 　　GUI Shu-hua, FU Tao, WANG Jie,et al. 　　New Drug RD Dept. in Guangzhou University of Chinese Medicine, Guangzhou 510006,China 　　? 　　【Abstract】 Objective To study the antifungal activity of extracts from Bark of Cleistocalyx operculateand Phyllanthus emblica L. in vitro for their application of medicinal pharmacology. Methods The minimum inhibitory concentration (MIC) and the minimum concentration (MFC) of extracts from Bark of Cleistocalyx operculateand Phyllanthus emblica L. against 11 strains dermatophytes were determined by media dilution method. Results Apart from Trichonphyton mentagrophyton (Wuhan strain) and Candida parapsilosis (Wuhan strain) (MIC=3.13μl/ml), MIC of Cortex Pseudolarici extract against other strains were 6.25μl/ml, MFC value were similar to its MIC. All the strains were not susceptible to extracts from Bark of Cleistocalyx operculateand Phyllanthus emblica L, their MIC50 μl/ml, miconazole nitrate?s MIC and MFC0.78 μl/ml. Conclusion Bark of Cleistocalyx operculate and Phyllanthus emblica L. have no antifungal activity against 11 strains dermatophytes.? 　　【Key words】 　　Cortex Pseudolarici； Cleistocalyx operculate； Phyllanthus emblica L;Antifungal activity; In vitro 　　? 　　土荆皮来源于松科植物金钱松(Pseudolarix kaempferi Gord)的干燥根皮和近根树皮。最早见于清代赵学敏所著《本草纲目拾遗》, 性辛、温、有毒、止痒杀虫, 用于治疗癣症。经研究发现, 其含有二萜类、三萜类、三萜内酯及甾醇类等成份。现代药理实验表明其具有抗真菌、抗肿瘤、抗生育、使胆囊自截等作用［1］。? 　　金钱松为我国二级保护植物，资源难求，在临床上用药治疗有一定的困难，因此水翁皮与余甘子皮常作为土荆皮的替代品在临床上使用。但对于水翁皮和余甘子皮是否真正具有抗真菌活性尚缺乏实验研究报道。本课题拟对作为“土荆皮”入药的习用替代药材水翁皮与余甘子皮进行抗真菌活性筛选研究，为水翁皮与余甘子皮的临床应用提供依据。?