B族链球菌核酸检测在产前筛查中可行性的研究.docVIP

B族链球菌核酸检测在产前筛查中可行性的研究 　　[摘要] 目的 探讨B族链球菌核酸检测在产前筛查中的可行性。方法 将2012年4月～2016年8月期间于我院产检的1000例孕妇作为研究对象，于35～37孕周期间采集孕妇肛周及阴道分泌物后做PCR检测与细菌培养，分别比较两种方法对B族链球菌的阳性检出率，随后分别抽取B族链球菌阳性与阴性各100例孕妇，比较两组的分娩方式、新生儿并发症、产妇并发症发生情况。结果 PCR检测方法的阳性检出率为8%（80/1000），明显高于细菌培养检测方法的阳性检出率4%（40/1000）；阳性组产妇产后出血、早产、胎膜早破及新生儿窒息、败血症、感染等并发症发生率均高于阴性组（P0.05）。结论 应用PCR检测方法能够提高B族链球菌的阳性检出率，尽早对B族链球菌为阳性的孕妇采取恰当的干预措施，可降低母婴并发症发生率，保证母婴健康平安。 　　[关键词] B族链球菌；产前筛查；可行性；研究 　　[中图分类号] R715.3 [文献标识码] A [文章编号] 2095-0616（2017）06-64-03 　　[Abstract] Objective To explore the feasibility of B-streptococcal nucleic acid detection in prenatal screening. Methods 1000 pregnant women tested in our hospital from April 2012 to August 2016 were selected as the subjects. After 35-37 gestational weeks， the perianal and vaginal secretions were collected for PCR and bacterial culture. The positive rate of B-type streptococcus was determined by the two methods. Then， 100 pregnant women with positive and negative B streptococcus were randomly selected. The delivery mode， incidence of neonatal complications and maternal complications were compared between the two groups. Results The positive detection rate of PCR was 8%（80/1000）， which was significantly higher than that of bacterial culture test （4%， 40/1000）. The positive group had postpartum hemorrhage， premature labor（P0.05）. The incidence of neonatal asphyxia， sepsis and infection is higher than that of negative group（P0.05）. Conclusion PCR detection method can improve the positive rate of B streptococcus， as soon as possible on the B group of streptococcus positive pregnant women to take appropriate intervention measures. It can reduce the incidence of maternal and child complications， to ensure maternal and child health and safety. 　　[Key words] Group B Streptococcus； Prenatal screening； Feasibility； Research 　　分娩?^程中，引起产妇与新生儿一并出现感染的病菌主要是B族链球菌（GBS）[1]，它属于革兰阳性菌，已有研究表明[2]，B族链球菌呈阳性和母婴结局、胎膜早破等现象有密切相关，检测孕妇B族链球菌是保证母婴健康、安全的重要手段[3]。早在20世纪90年代，CDC便制订了B族链球菌的检测、筛查及处理指南[4]，AAP、ACOG提倡怀孕35～37周的孕妇进行B族链球菌的产