三桠乌药天然群体种子表型多样性的研究.docVIP

三桠乌药天然群体种子表型多样性的研究 　　摘要：为提高三桠乌药种质资源保护与利用成效，以河南、山东天然分布的7??三桠乌药群体的种子为材料，对其5个表型性状进行方差分析、相关分析及聚类分析。方差分析结果表明，三桠乌药种子表型性状在群体间和群体内均存在丰富变异，MP1天然群体种子的长度、宽度、体积和表面积与其它6个天然群体的相应性状均存在差异，且达到极显著水平；LS群体与MP1、MP2群体，MP1群体与其它6个群体的种子体积也均存在极显著差异。相关性分析结果表明，7个三桠乌药天然群体种子的5个表型性状与其采集地的海拔、经纬度、年均温和年降水量均存在一定相关性。聚类分析结果表明，在距离为2时，可以明显将7个群体分成4组：第一组包括MP1，第二组包括LS，第三组包括QX、MP2和MP3，第四组包括ZY和LC。昆嵛山MP1和崂山LS天然群体资源对整个三桠乌药种质资源的保护和创新利用具有重要价值。 　　关键词：三桠乌药；种子；天然群体；表型多样性 　　中图分类号：S792.230.4文献标识号：A文章编号：1001-4942（2018）02-0019-05 　　Abstract To improve the efficiency of conservation and utilization of the germplasm resources of Lindera obtusiloba，the variance analysis， correlation analysis and cluster analysis were carried out on five phenotypic traits of the seeds of 7 L.obtusilobanatural populations distributed in Henan and Shandong provinces. The analysis of variance showed that there was abundant variation in the phenotypic traits of seeds between and within populations. The length， width， volume and surface area of MP1 seeds were extremely significantly different from those of the other six natural populations. The seed volume of LS was significantly different from that of MP1 and MP2， and that of MP1 was also significantly different from that of the other six natural populations. The correlation analysis showed that the five phenotypic traits of the seven natural populations of L. obtusiloba had certain correlations with altitude， latitude and longitude， annual mean temperature and annual precipitation. The cluster analysis showed that the seven populations could be divided into four groups at the distance value of 2： the first one included MP1， the second one included LS， the third one consisted of QX，MP2 and MP3，and the fourth one included ZY and LC. The natural populations of MP1 from Kunyu Mountain and LS from Laoshan Mountain have important values for conservation and innovation of L. obtusiloba germplasm resources. 　　Keywords Lindera obtusiloba；Seed；Natural population；Phenotypic diversity 　　三桠乌药（Linder