草鱼Ⅰ型胶原蛋白α1基因cDNA全序列克隆组织分布及其-水产学报.PDF

36 6 Vol.36, No.6 2012 6 JOURNAL OF FISHERIES OF CHINA June, 2012 文章编号: 1000-0615(2012)06-0849-10 DOI:10.3724/SP.J.1231.2012.27768 草鱼 Ⅰ型胶原蛋白 α1 基因 cDNA 全序列克隆、组织分布及其 生物信息学分析 1,2 1 1 1 1* 1 1 , , , , , , (1. , , 510380; 2. , 201306) : PCR RACE Ⅰ α1 (COL1A 1) cDNA , 5 772 bp, 4 347 bp, 1 448 BLAST , COL1A 1 , 93.90% 93.60%, , , , COL1A1 137.2 ku, 5.44, αβ18 , 22 , 17 COL1A1 33~69, 1 249~1 355 62~104 RT-PCR ,COL1A 1 8 , mRNA 4 (P0.05) : ; Ⅰ; COL1A1; ; : Q 781; S 917 : A Ⅰ(type Ⅰcollagen) , (collagen family) , [13-15] , , PCR RACE COL1A 1 , cDNA , [1-12] , α1 PCR COL1A 1 (COL1A1) , COL1A1 , COL1A 1 [7-8], COL1A1 [9] 1 (Ctenopharyngodon idellus) (Cypriniformes) (Cyprinid) 1.1 (Leuciscinae), 2009 2 ,