补肾益气方对家兔koa软骨组织中aqp3表达的影响中西医结合临床专业论文.docxVIP

The experimental study on expression of AQP3 in the articular cartilage of rabbit knee osteoarthritis by Bushenyiqi formula treatment Abstract Objective: To study the effect on contents of AQP3, and change of histomorphology of rabbit knee osteoarthritis cartilage by Bushenyiqi Fordula treatment and observes its curative effect. Method: The forty Japan big ear rabbits male are limited, the weight approximately 2.50±0.10kg, were divided into 4 sets randomly：Normal set(A), model set(B), control set(C), treatment set(D), each 10. Indiscriminating condition breeding one week, normal set(A) with no treatment, rest sets adopt plaster cast builds aosteoarthritis mold. Since building aosteoarthritis molds, Normal set(A) applying nothing; physiological-saline to model set(B); glucosaminehydrochloride to set(C); Bushenyiqi Formula to set(D).After 4 weeks treatment, execute rabbits and gather cartilage to detect contents of AQP3,observation pathological change of cartilage. Result: Experiment studies have shown that the control set and treatment set have significant knee cartilage degeneration than model set. they have significant difference to compare with model set （ P ＜ 0.05）.Between treatment set and control set, contents of AQP3 have no significant different in articular cartilage（P＞0.05）. Conclusion: Bushenyiqi Formula had preventive and therapeutic action in rabbit knee osteoarthritis. One of its mechanism is considered as decreasing contents of AQP3 to enhance arthrodic cartilage matrix, inhibit from its degrading, antagonism inflammatory factor katogene and limit joint cartilage matrix further harm and protect joints. Keywords: KOA; Bushenyiqi Formula; Cartilage; AQP3 Author：Wu Yu Tutor： Liu Deyu 目 录 HYPERLINK \l _bookmark0 引 言 1 HYPERLINK \l _bookmark1 文献综述 3 HYPERLINK \l _bookmark2 .现代医学对 KOA 的认识 3 HYPERLINK \l _bookmark3 1.1 KOA 的概念与流行病学 3 HYPERLINK \l _bookmark4 1.2 KOA 的病因和分类 3 HYPERLINK \l _bookmark5 1.3 现代医学对 KOA 发病机理的研究 4 HYPERLINK \l _bookmark6 1.4