多模态反射荧光实时共聚焦成像系统的研制-生物医学工程专业论文.docxVIP

第 第 II 页 本文工作的具体内容包括以下几个方面： 1 多模态反射荧光实时共聚焦成像系统硬件部分实现，包括反射光 路和荧光光路设计、扫描装置设计、检测装置设计。 2 多模态反射荧光实时共聚焦成像系统软件部分实现，包括扫描方 式算法、采集方式算法、图像重建算法以及对该系统的一系列优化算法 的实现。 3 多模态反射荧光实时共聚焦成像系统在皮肤组织成像中的应用， 包括表皮结构的反射成像实验、黑色素瘤诊断实验。 关键词：共聚焦，反射，荧光，医学成像，皮肤病学 第 第 III 页 THE DEVELOPMENT OF MULTIMODALITY REFLECTANCE AND FLUORESCENCE CONFOCAL MICROSCOPE ABSTRACT Confocal laser scanning microscopy has been widely accepted in dermatological research, due to its advantages in non-invasive, real time, high resolution in vivo imaging. Conventional confocal laser scanning microscopy can be divided into two modes: reflectance mode and fluorescence mode. The reflectance mode demonstrates tissue structural information based on the different refractive indices among the various tissue microstructures. The fluorescence mode can detect tissue pathological information with the excitation of exogenous fluorescent dye to produce contrast. However, up to now most researchers have been focusing on either reflectance or fluorescence confocal imaging separately which can only provide either structural or pathological information of tissue. This greatly affected the accuracy of dermatological diagnosis by lack of enough diagnostic information. In order to improve diagnostic performance of dermatosis efficiently, we developed a new design of noninvasive simultaneous multimodality fluorescence and reflectance confocal microscope which combines the 第 第 PAGE IV 页 structural imaging and pathological imaging. Moreover, in the confocal system the fluorescence imaging is aim to autofluorescence substance such as nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) which exclude the toxic affection from the introduction of exogenous fluorescent dyes. With both imaging modality acquired simultaneously, the combined image revealed the morphological and pathological information of the suspicious site, yielding a possibility of precancer diagnosis of skin. The study contents in this thesis are de