中华倒刺同工酶组织特异性研究.docVIP

中华倒刺同工酶组织特异性研宄 运用聚丙烯酰胺凝胶垂直板电泳技术， 对中华倒刺？的6种组织（脑、眼睛、心脏、肝脏、肾 脏、肌肉）中的乳酸脱氢酶（L D H）、苹果酸脱氢酶 （MDH）、酯酶（E S T）进行了初步研宄，并对这 3种酶在各组织中的同工酶位点及酶谱表型进行了分 析。结果表明，中华倒刺？的3种同工酶系统具有组织 特异性。LDH检测到经典的5条谱带，由3个基因 位点编码，C位点仅在肝脏组织中表达；MDH在5 种组织（除肌肉外）中检测到3条酶带，仅为细胞质 型，肌肉组织中同时表达了细胞质型和线粒体型；E ST至少由5个基因位点编码，同工酶酶谱复杂，肝 和肾的活性表达较强。 关键词：中华倒刺？；同工酶；组织特异性 S 9 6 5 . 1 9 9 A :0439- 8 114 （ 2 0 11） 24—5206-05 Study on the Isozymic T issue Specificity of Spi nibarbus sinensis ZHANG Juanl， 2， WANG Hon g — yel，2，CAI Yan—zhi2，LU T a o 3 , QIN Xiao — yan3 (1 . C o 1 1 e g e of Life Scie nee, Huazhong Normal U n i v ersity， Wuhan 4 3 0 0 7 9, Chi n a ； 2 . Fisheries Research Institute of Hubei Provi nee, Wuhan 430071， China； . Zigui Governing Statio n for Fishery Admi strati on，Vessel Checking and P o r t Supervision, Zigui 44 3600， Hubei, China) Abstract： By using the vertical polyacrylamide gel electrophoresis, I a c t ate dehyd rogena s e (LDH), m a late dehydrogenase (M D H ) and esterase (EST) isozym e s from six tissues(brain eye, heart, liver， kidney and muscle) of adult Spin ibarbus sinensis were p r eliminarily studied. The loci and phenotypes of t h e isozymes in every tissu e were also analyzed. The results showed that all i sozymes presented tissue specificity. Five classi cal L D H bands were found in the tissues, which were e ncoded by three loci, and the site C was only found in liver. The number of M D H isozyme bands was three in five tissues except mu s c 1 e , which were all s—MD H . Both s —M D H and m —M D H we re found in muscle. E S T is ozyme was encoded by five loci at least. The isozym e pattern was complicate d， and the stronger a c t i v ity was pigmented in the liver and kidney. Key words： Spinibarbus sinensis； isozyme； tissu e specificity 同工酶的概念最早是由Ma r k e r t和Mo 1 1 e r [ 1 ]在1 9 5 9年提出，是指催化相同化学 反应，但其性质不尽相同的一类酶，它是普遍存在的， 在同一物种的不同个体或同一细胞的不同部位，如细 胞膜、细胞质和线粒体等细胞器，以及生物生长发育 的不同时期和不同代谢条件下，都有同工酶的存在， 这类酶对细胞的发育及代谢调节都很重要。同工酶是 由染色体上不同的基因位点或同一位点的等位基因编 码的，是基因型的生化表现型，反映了编码酶蛋白的 DN A序列信息。同工酶能催化相同的反应，是因为