创伤性关节炎关节滑液中炎性细胞因子的表达及意义的临床分析-外科学(骨外)专业论文.docxVIP

Abstract Objective: Purpose of the study is to detect the concentration of TNF-α and IL-1 in the synovial fluid of PTA ． To observe the relationships between both TNF-α 、 IL-1 and knee post-traumatic arthritis X-stage ( Kellgren Lawrence criteria) ． To investigate the internal relationships between inflammatory cytokines and the severity of knee PTA，and to search biochemical indicators to reflect the degree of PTA． Material and method： We selected randomly 40 patients （21 males and 19 females， 18-30 age）with knee PTA from our outpatients and inpatient in 2014(since January to June) ． The patients according to X- stage (Kellgren Lawrence criteria) were divided into 2 groups (light and heavy)，20 patients in each group，extract effusion of knee joint ,measured TNF-α and IL-1 potency．In addition to randomly select 10 healthy volunteers (no other knee joint disease) the same extraction knee joint fluid inspection as control, Of which 6 were male, 4 female, age 19-32 years old, average age is 24.59 years old l. Adopt enzyme-linked immunosorbent assay (ELISA) was detected TNF-α and IL-1. Applying SPSSl6.0 statistical analysis software, t test, the concentration of experimental observation of TNF-α and IL-1 in each group has no statistical difference. And using Pearson correlation analysis to analyze the relationship between concentration and PTA X-ray grading of TNF –α and IL-1. Result: Liquid TNF- α and IL-1 concentrations of knee traumaticarthritis were higher than those in the control group, the difference was significant (P 0.05) .Each group of knee traumatic arthritis TNF- α and IL-1 concentration with X-ray stage increased, and there were significant differences (P0.05). Conclusion： Aggravated knee traumatic arthritis synovial fluid of patients with TNF-α and IL-1 concentration increased gradually with the disease. X-ray concentration and PTA TNF- α and IL-1 staging is closely related to the severity of the concentration of PTA, su