创伤性变色牙的变色机制及漂白疗效研究-口腔临床医学专业论文.docxVIP

中文摘要 目的:本实验旨在探讨创伤性变色牙的变色机制及漂白疗效。 材料与方法:在 20 颗因正畸拔除的健康离体牙髓腔内封入新鲜红细胞，以 2500rpm/min 离心 25 分钟，每天两次，连续离心 3 天制备变色牙模型。将变色牙随机分 为 A 、B 两组，每组 10 颗，对 B 组牙使用 10%过氧化服凝胶进行冠内、外联合漂自主 连续漂白四周 A 组为对照组。 A 、B 两组牙最后依序经 EDTA 脱钙、梯度酒精脱水、正 丁醇透明、石蜡包埋、组织切片及一系列组织化学染色处理，以分析牙齿内红细胞的分 解程度及漂臼前后化学成分的变化。囚种组织化学染色方法分别是:联苯胶实验〈检测 高铁血红紊);铮自实验〈检测血红蛋白头 PerlS 实验〈检测铁离子); Turnbull 蓝实验 〈检测含铁血黄素〉 结果z 所有实验牙经过三天连续离心后均变色。组织化学实验结果显示:变色牙牙 本质小管内存在高铁血红索和血红蛋白 p 缺乏铁离子和含铁血黄素:漂白后的牙本质小 管内未见任何红细胞的分解产物。 B 组变色牙漂白 4 周后染色均消失 F 牙齿颜色恢复正 常。 结论:在无菌条件下 F 创伤性牙齿的变色是由血红蛋白和其他形式的高铁血红素分 子所导致的 F 过氧化物漂白剂能有效与之作用而产生漂白效果。 关键词:创伤性变色牙组织化学漂白 Abstract Objective: To investigate the mechanism of discoloration and the efficacy of bleaching of traumatized teeth. Materials and methods: Vitro model of discolored teeth: Sample of fresh erythrocytes was placed in the pulp cavity of 20 extracted teeth and centrifuged twice daily for 25 minutes over a period of 3 consecutive days.These discolored teeth were divided equalIy and randomly into two groups .The teeth of Group B were bleched internaIly and externally with 10% carbide peroxide gel for. 4 weeks.The teeth of group A is control group. And then all teeth were prepared for histological examination and subjected to a series of histochemical test : (1) benzidine ，(2)zinc leuco，(3)Perls and (4) Tumbull Blue to analyse some of the biochemical changes fol1owing haemorrhage into the pulp. chamber and postbleaching. Results: Haemoglobin and haematin were det巳cted in the dentinal tubules of discolored teeth from group A and no evidence of ferric or haemosiderin. Specimens from group B demonstrated .a negative response to histochemical tests. Conclusion: In the absense of bacterial invasive ，it is haemoglobin and haematin that cause discoloration of traumatized teeth. Peroxide bleaching gel can e筐ectively get rid of haemoglobin and haematin. Key Words: traumatized teeth discoloration bleaching histochemical tests u 郑重声明 本人的学位论文是在导师指导下独立撰写并完成的声学位论文没有到 窃、抄袭、造假等违反学术道德、学术规范和侵权行为，本人愿意承担由此 而产生的法律责任