从水通道蛋白亚型(AQPs)的定性定量表达研究湿困脾胃证的水液代谢机制-中医诊断学专业论文.docxVIP

ABSTRACT Purpose: Comb theoretical knowledge of the syndrome of Dampness Blocking Spleen and Stomach， study the qualitative quantitative expression characteristics of subtypes (AQPs) water channel protein , part reveal the pathology mechanism of moisture trapped spleen and stomach syndrome and flat stomach powder treatment effect mechanism. Methods: Through literature research, combing the characteristics of wet trapped spleen and stomach syndrome and the treatment regularity of drug joint the inside and outside wet factor together（ lived wetlands for a long time, excessive wet outside;improper diet, affect-mind dissatisfaction，excessive wet inside), established the subjects of wet spleen and stomach syndrome model by SD rat. using immunohistochemical (IHC) detection of AQPs in the digestive tract anatomy of the related parts of the distribution and expression, enzyme league immune method to determine the content of gastric tissue AQPs (ELISA). Result: Wet trapped taste card to be born inside wet chaotic, poor angry machine, coke taste trapped as the main cause; To taste the dirty as the main disease; Has given priority to with wet, in the disease of sex is easy and its evil, can characteristics of thermalization cold; In an attempt to physical contrast with the forces of evil are related, more touching refractory characteristics; On syndrome, wet as its height pixu (spleen deficient) as the main performance, has a certain space and time characteristic; On the treatment for wet pixu (spleen deficient) the main pathogenesis, ingredients of spleen qushi more genera. AQP0, 1, 2, 3 main expressed in gastric tissue mucosa layer, outer membrane expression may have suspected, little expression in submucosa and muscular layer. QP0, 1, 2, 3 have differences in distribution in the gastrointestinal tract. Under normal circumstances (blank), AQP0 mainly expressed in the middle of the gastric cardia and gastric body, compared with blank group,model group has a tendency to c