房县小冬菇中香菇多糖的精制、结构鉴定和体外抗肿瘤活性分析-药剂学专业论文.docxVIP

Part two The structure of LNT1 and LNT2 The chemical structures of LNT1 and LNT2 were studied by Acid hydrolysis、 Periodate oxidation 、 Smith degradation 、 Methylation analysis 、 1D and 2D NMR (DQF-COSY, HSQC, and HMBC) experiments. The chain conformation of LNT1 and LNT2 were evaluated by Congo Red Test. Analysis of monosaccharide composition showed that LNT1 and LNT2 only contain D-glucose. Periodate oxidation-Smith degradation Test showed that LNT1 and LNT2 contain (1-3) or (1-3-6), (1-6) or terminal sugar resides. Congo Red Test showed that LNT1 and LNT2 exsits as triple-helix in distilled water and a helix-coil transition may occur when NaOH concentration is higher than 0.20M. Results from FT-IR showed that LNT1 and LNT2 wereβ-D-Glycopyranoside polysaccharides. On the basis of Periodate oxidation、Methylation analysis、1D and 2D NMR (DQF-COSY, HSQC, and HMBC) experiments the polysaccharides (LNT1 and LNT2) were shown to consist of repeating units with the following structure. β-D-Glp 1 ↓ 6 →3) -β-D-Glp-(1→3) -β-D-Glp- (1→3)-β-D-Glp-(1→ Part Three The cytotoxicity assay of LNT1 and LNT2 The cytotoxicity assay of LNT1 and LNT2 on H-22 tumor cells were evaluated in vitro using MTT assay. The growth of H-22 tumor cells could be inhibited by LNT1 and LNT2 at a concentration as low as 12.5 μg/mL, and the inhibition ratios are 24.38% and 27.52%, respectively, and a dose-dependent manner was observed up to 800 μg/mL. LNT1 and LNT2 exhibited strongest inhibition ratio against H-22 tumor cells at the concentration of 800 μg/mL, reached 52.31% and 52.62%, respectively. So LNT1 and LNT2 have the value to further study. [Key Words] Lentinan; alkaline extraction technology; chemical structure; NMR; antitumor activity in vitro. 独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保 留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查