生化2018 c21 gene recombination and recombination technology教学材料.ppt

(1) The preparation of target DNA It represents whole DNA sequence of a genome ① To find a fragment from genomic library Genomic library contains a comprehensive DNA fragments from genomic DNA cut by specific RE. During the construction of a genomic library, DNA fragments and their vectors are ligated, and then introduced into recipient cells. ② To prepare from cDNA library or cDNA Extracting total mRNA Reverse transcription Ligation It represents the population of mRNAs coding for genes and protein expression Transformation Proliferation Abundant Clones ③ To prepare the gene fragment