溃疡性结肠炎大肠粘膜组织IL33 mRNANFκB1 p50 mRNA表达及其相关性的研究.docVIP

溃疡性结肠炎大肠粘膜组织IL33 mRNANFκB1 p50 mRNA表达及其相关性的研究 　　摘要：目的 了解在UC组织中IL-33与NF-κB mRNA的表达和相互关系，探讨NF-κB、IL-33在参与UC发生、发展过程中的可能作用。方法 选择UC活动期患者30例，缓解期25例，正常健康人30例。应用实时荧光定量RT-PCR方法检测NF-κB1 p50 mRNA、IL-33 mRNA在上述各组大肠粘膜组织的表达量。结果UC活动期组NF-κB1 p50 mRNA和IL-33 mRNA在大肠粘膜组织相对表达量显著增高，分别与UC缓解组及正常对照组比较，差异均有显著性（P0.05）。相关分析结果显示，NF-κB1 p50 mRNA和IL-33 mRNA两者在UC活动期大肠粘膜组织的表达量呈正相关(r=0.834，P0.01)；NF-κB1 p50 mRNA和IL-33 mRNA在UC活动期大肠粘膜组织的表达量分别与评估UC活动性的改良Mayo评分呈正相关(r=0.882，P0.01；r=0.811，P0.01)。结论 NF-κB1 p50 mRNA、IL-33 mRNA均参与UC的发生、发展过程；NF-κB 与IL-33 相互作用，参与UC的炎症活动。 　　关键词 溃疡性结肠炎；NF-κB1 p50 mRNA；IL-33 mRNA；实时荧光定量-PCR 　　 　　Research on the correlation of IL-33 mRNA and NF-κB1 p50 mRNA in ulcerative colitis 　　Abstract：ObjectiveTo investigate the relationship between NF-κB1 p50 mRNA and IL-33mRNA in UC, by testing their expression in the colonic mucosal of patients with UC, and discuss whether there is correlation between NF-κB and IL-33 in the UC and the pathogenesis of NF-κB p50 mRNA and IL-33mRNA in the occurrence and development of the ulcerative colitis.Methods Colonic mucosal biopsies from the colon of patients with 30 active UC patients,25 inactive UC patients, and 30 healthy controls. The expression of NF-κB1 p50 mRNA and IL-33 mRNA in colonic mucosal were detected by RFQ -PCR.Results The expression of NF-κB1 p50 mRNA and IL-33mRNA significantly increased in active UC group compared with inactive UC group and healthy control group respectively(P＜0.05), Correlation analysis displayed that NF-κB1 p50 mRNA and IL-33 mRNA were positive correlation in active UC tissues.(r=0.834，P0.01). NF-κB1 p50 mRNA was positively correlated with the modified Mayo Score (r=0.882，P0.01) , the same as IL-33 mRNA (r=0.811，P0.01).Conclusion Both NF-κB1 p50 mRNA and IL-33 mRNA involve in the process of occurrence and development of UC. They had a hand in the inflammation of UC. 　　Key words Ulcerative colitis(UC); NF-κB1 p50 mRNA; IL-33 mRNA; RFQ- PCR 　　 　　溃疡性结肠炎（ulcerative colitis，UC）是一种慢性非特异性肠道炎症疾病，其发病机制尚未十分明了。NF-κB