临床医学毕业论文h2s对兔离体心脏心率的影响.docVIP

H2S对兔离体心脏心率的影响 姓 名： 2014年6月25日 H2S对兔离体心脏心率的影响 ：陈立锋徐涛范凌赵兰平陈少峰温晓竞张晓云马建 伟尹万斌 】 0的：探讨硫化氢(H2S)对兔离体心脏心率的影响。方法：用标 准微电极细胞内记录技术，观测硫化氢对兔右心房心肌细胞动作电位频率的影 响。观测指标：自发放电频率(rate of pacemaker firing, RPF)。结果：(1)用 200(imol/LNaHS灌流时，和正常对照组相比，RPF显著减小(P0.01)。(2) 400gmol/L NaHS 与对照组比较，RPF 显著减小(P0.01)。和 200(imol/LNaHS 相比，RPF显著减小(P0.01)。⑶以上使RPF减小的效应在NaHS灌流3?5min 出现，lOmin时己经有显著减小(P0.01), 25?30min达到高峰。结论:H2S可 浓度依赖性的影响兔离体心脏的心率。 关键词】心率；电生理；硫化氢；心脏；兔 Hydrogen su!fide(H2S)has been proved to be the third endogenous signaling gasotransmitter，besides nitric oxide(NO) and carbon monoxide(CO).It shows important physiological functionsjnvolving in the long term potentiation in the hippocampus in the central nervous system,relaxing gastrointestinal tract smooth muscles[l，2].H2S has been also found to play many regulating functions in cardiovascular system, and play a certain effect in many cardiovascular diseases[3].But the effect on heart rate hasn t been deeply reseached.By using standard intracellular microelectrode technique to record Aps，hydrogen sulfide was used to investigate the rate of pacemaker firing of left atrium.The aim of the study was to observe the effects of hydrogen sulfide on heart rate of rabbit hearts in vitro,elucidate the mechanism(s) involved,provide electrophysiologic proof for the nosogenesis of some cardiovascular diseases and provide research directions for the development of cardiovascular drugs. MATERIALS AND METHODS Animal and Reagents Rabbits of either sex(2.2±0.2)kg,provided by Experiment Animal Center of HeBei North University.NaHS was prepared with HC1 and Na2S after the experiment began. Specimen Preparation Rabbits were killed with a single blow on the head and the hearts were quickly removed and placed in warm(36.0土0.5) °C improved Locke solution(NaCl 0.157mol/L,KCl 0.56x10-2mol/L,CaCl 0.21x10-2mol/L,NaHCO30.18x10-2mol/L and glycose 0.5610-2mol/L).It was oxygenated with 95%O2 and 5%CO2 and maintained at ( 36.0士0.5 ) °C with pH of 7.40士0.03.Thc right atrium myo