高效液相色谱法同时检测胶基糖果中的7种糖醇-食品安全质量检测学报.PDF

7 5 Vol. 7 No. 5 2016 5 Journal of Food Safety and Quality May , 2016 7 * 陈树东 , 林晓佳, 林森煜, 刘海虹, 严婉盈, 吴钟玲 (, (), 511447) 摘 要: 目的 -(HPLC-RID) , 7 方法 Phenomenex HPX-42C , , , 结果 7 (Van’t Hoff), ; 50~5000 mg/L , (r2)0.999 , 25 mg/kg, 94.4%~108%, 0.95%~7.3%结论 , 关键词: ; ; Simultaneous detection of 7 kinds of sugar alcohols in the gum base candy by high performance liquid chromatography * CHEN Shu-Dong , LIN Xiao-Jia, LIN Sen-Yu, LIU Hai-Hong, YAN Wan-Ying, WU Zhong-Ling (Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou 511447, China) ABSTRACT: Objective To develop a high performance liquid chromatography with refractive index detection (HPLC-RID) method for the simultaneous determination of maltitol, ribitol, glycerine, mannito, lactitol, xylitol and sorbitol in gum based candy. Effects of temperature and flow rate on the separation efficiency of 7 kinds of sugar alcohols were investigated and optimized. Methods Using Phenomenex HPX-42C column with refractive index detector, ultra pure water as mobile phase, the content of sugar alcohols were determi