氮对粗壮女贞叶片生理特性及内含物含量影响.docVIP

氮对粗壮女贞叶片生理特性及内含物含量的影响-园林 氮对粗壮女贞叶片生理特性及内含物含量的影响 doi：10.13360/j.issn.1000-8101.2015.04.008中图分类号：S718.43 葛敏凯1，王德炉1*，周云超1，何滔2 （1.贵州大学林学院，贵阳 550025；2.贵州省湄潭县林业局） 摘要：通过砂培试验，研究了5个供氮水平对粗壮女贞叶片生理特性以及叶片内含物含量的影响。结果表明：氮浓度为5.8 mmol/L时，粗壮女贞氮素不足，植株叶绿素总量最低，植株生长受到抑制，但叶片内含物含量较多；而氮浓度为15.0 mmol/L时，与其他处理相比，粗壮女贞氮素充足，整体生理机能良好，生长速率较高，叶片中游离氨基酸含量较高，但水浸出物和皂甙含量最低，叶片中其他内含物含量也受到了一定影响。 关键词 ：粗壮女贞；氮；生理特性；叶片内含物；苦丁茶 Effects of nitrogen on some physiological characteristics and inclusion contents in leaves of Ligustrum robustum ∥ GE Minkai， WANG Delu， ZHOU Yunchao， HE Tao Abstract：We studied separately the effects of five levels of nitrogen treatments on physiological property and inclusion contents in the leaves of Ligustrum robustum in a sandy culture experiment.These results showed that under the 5.8 mmol/L nitrogen treatment， the chlorophyll content was the lowest， and the plant growth was restrained because the nitrogen was not enough for the L. robustum， but the inclusion contents in leaves were much higher. Under the 15.0 mmol/L concentration of nitrogen treatment， the physiological function was well， which led the plant to a much higher growth rate with more free amino acid content in the leaves， because the nitrogen was sufficient for the plant. But the aqueous extracts and saponin contents in leaves were the least， the other inclusions except for aqueous extracts and saponin were also influenced. Key words：Ligustrum robustum； nitrogen； physiological characteristics； inclusion contents in leaves； kuding tea First author’s address： College of Forestry， Guizhou University，Guiyang 550025，China 收稿日期：2015-01-29 修回日期：2015-05-09 基金项目：贵州省农业科技攻关项目（黔科合NY字［2007］3040号）。 作者简介：葛敏凯（1989-），男，硕士生，研究方向为林木栽培生理生态与分子生物学。通信作者：王德炉，男，教授。Email： fc.dlwang@ gzu.edu.cn 在农业生产中，氮肥是增产效果最为显著的一类化学肥料，它对提高作物产量，改善农产品质量有十分重要的作用［1］。但不合理的使用速效氮肥会造成其利用率明显降低，未被利用的氮素中小部分仍保留在土壤中，大部分通过硝化和反硝化作用，以NO3-和N2O形式排放到环境中造成污染［2］。粗壮女贞（Ligustrum robustum）属木犀科植物，其叶片制成的代茶饮料俗称苦丁茶［3］，含有许多生物活性成分和抗氧化物质［4-5］，具有良好的保健功