致病三大弧菌知识讲稿.pptVIP

Studies on vibrio parahaemolyticus by PCR during the four seasons in the south coast of taiwan;V. parahaemolyticus;TCBS V. parahaemolyticus : 直徑1~2mm、有光澤、綠色 V. cholerae : 直徑2~4mm 、平滑、黃色、中心不透明、周圍透明 嗜鹽性試驗(0% 、 1% 、 6% 、 8% 、 10%) V. parahaemolyticus : 6% 、 8%生長良好 V. cholerae : 0% 、 1%生長良好;Vibrio parahaemolyticus is one of the most important food-borne pathogens in Taiwan, Japan and other coastal countries. Vibrio parahaemolyticus is a gram-negative, halophilic bacterium that occurs naturally in estuarine environments world-wide. (Environmental Microbiology 5(8),706-710 ,2003 );Thermostable direct hemolysin (TDH) TDH/ TDH-related haemolysin (TRH) Urease positive Lethal toxin 附著因子 ;Pathogenic V.parahaemolyticus generally produces a thermostable direct hemolysin (TDH). More than 90% of clinical V.parahaemolyticus isolates, but fewer than 1% of food or environmental strains produce TDH. (APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2003) ;研究架構;採樣地點;採樣方法;水質測量;Sample collection (3水樣3有機樣本) APW 每管APW各取1loop接種於TCBS 抽取DNA 挑選典型綠色菌落分別各接種於TSB-2及TSA-2 PCR(toxR) 由TSB-2 抽取DNA PCR (toxR) toxR(+): toxR(-): PCR (tdh) PCR (16S rRNA) toxR(+) PCR (trh) 致病性分析 生化鑑定 菌體抗原 PCR (tdh) KP試驗 PCR (trh)