《盐藻SOD分子特性研究》-毕业论文.docVIP

本 科 毕 业 设 计 (论 文) 盐藻SOD分子特性研究 Study molecular properties of SOD from Dunaliella salina 学 院： 海洋学院 专业班级： 生物工程 生工 学生姓名： 学 号： 指导教师： （教授） 毕业设计（论文）中文摘要 盐藻SOD分子特性研究 摘 要：超氧化物歧化酶是生物体防御氧化损伤的一种主要酶，在临床治疗和预防、食品、化妆品等领域应用广泛。本实验对盐藻SOD的分子特性进行了初步研究。实验取培养至对数期的盐藻细胞，通过反复冻融和超声波破碎获得SOD原酶液，加入-35℃的丙酮进行沉淀后，再在70℃水浴条件下热处理10min，纯化倍数可达11.30。该纯化酶液经SDS-聚丙烯酰胺凝胶电泳，检测出一条SOD谱带，与标准蛋白比对得知，其分子量为23.1KD。通过波谱对纯化出的SOD进行扫描，检测到其紫外最大吸收波长为259nm，可见光区最大吸收峰波长为671nm。通过加入不同浓度的H2O2，明显发现样液中SOD活性受到抑制。结合现有资料判断确认，盐藻中的SOD应为Cu/Zn-SOD类型。 关键词：SOD；盐藻；分子特性 毕业设计（论文）外文摘要 Study of molecular properties of SOD from Dunaliella salina Abstract: Superoxide dismutase is the organism oxidative damage defense of one of the main enzyme. In clinical treatment and prevention, food, cosmetics and other fields are widely used. In this experiment, the molecular properties of SOD Dunaliella salina was studied. Experimental training to take the logarithm period, the cell through repeated freeze-thaw and ultrasonic broken obtain SOD enzyme, join - 35 degrees of acetone precipitation were at 70 degrees heat water purification 10min, under the condition of 11.30 multiples. The purified enzyme liquid through SDS- polyacrylamide gel electrophoresis, detection a SOD spectrum, and the standard that the molecular weight than protein for 23.1 KD. Through the spectrum of purification of SOD, detect the ultraviolet absorption wavelength 259nm for maximum absorption peaks, visible wavelength area biggest for 671nm. By adding different concentrations of H2O2, obviously found samples of suppressing SOD activity. Combine existing material, the judge should Dunaliella salina SOD was Cu/Zn - SOD type. Keywords: SOD；Dunaliella salina；molecular properties  目 录 1 绪论………………………………………………………………………………1 1.1 盐藻的简介……………………………………………………………………1 1.2 杜氏藻开发应用的现状和前景……………………………………………2 1.3 超氧化物歧化酶(SOD)的概况……………………………