荸荠组织培养-研究.pdfVIP

③ From 18℃ to 32℃, the propagation coefficient was rising with temperature rising and the numbers of foliar stem of average fleshy stem was rising at first, then descending later. 25 ℃ was favorable to subculcture of Chinese water chestnut cluster buds. Its propagation coefficient was 4.32, and average numbers of foliar stem per fleshy stem was 2.12. The buds and stem growth best at this temperature. So the favorable temperature and pH to propagation of cluster buds were separately 25 ℃and 6.0. The favorable medium was MS+6-BA0.5mg/L+KT0.5mg/L+NAA0.01mg/L +3%sucrose. （2 ）The kinds of plant growth regulators and their compounding, the kinds of different explants, light conditions had different effects on the callus induction. The result indicated that 2 ，4-D was the key factor for callus induction. The cytokinin (6-BA、KT 、ZT) could improve inducted effect of 2,4-D.Its sequence was that: 6-BA ＞KT ＞ZT.The favorable medium to induce callus by using fleshy stems as explants was MS+2,4- D1mg/L+6-BA0.5mg/L +3%sucrose +0.7%agar with 88.19% inductive rate.The inducted rate of fleshy stems was the highest one in all kinds of explants.The inducted rate of callus in the light power of 0 Lux was higher than that in 120 Lux or 2000 Lux. The result showed that the lower concentration level of 2, 4-D is advantageous to the propagation and differentiation of callus . Lingfasu is advantageous to callus differentiation .TDZ has the very good function to maintain the vigor of callus and prevente the callus aging . （3 ）The root induction of Chinese water chestnut was easy. The inducted rates of four mediums were all high and their difference were not marked. But the difference of their root number of average plantlet was marked. The favorable medium was