水杨酸与硫代甲硫氨酸对大豆生长的影响-嘉南药理大学图书馆.PDFVIP

理 硫硫 類 CNAL92-07 行92 年1 1 92 年12 31 麗 參 行 年 (Glutamine synthetase GS,EC 6.3.1.2) 老硫硫 (Methionine sulphoximine MSO)GS Salicylic acid SA 度 SA 來刺 理狀 例SA 理SA 金 SA 理 GS 論SA MSO 論MSO 理SA 理 兩不類 老PCD SA MSO 葉SA 度 MSO 立 Effects of Salicylic acid and MSO on Soybean Growth and Development Abstract Glutamine synthetase (GS) is a key enzyme in N metabolism and it catalyses the synthesis of Glutamine (Gln) from glutamic acid, ATP, and NH4+ . L-Methionine sulfoximine (MSO) is a glutamate analogue, it is also the GS inhibitor. Salicylic acid (SA) is an endogenous growth regulator of phenolic. At the same time, SA can induce a protective effect on plants under stress. Convincing data have been obtained on the SA-induced in the resistance of barley seedlings to heavy metal and herbicide. This research is divided into two parts to confer what influences on soybean treated by MSO and SA. First, we demonstrate the effects on soybean seeds treated by MSO alone according to the expression of GS gene, including GS activity and GS biosynthesis. Second, we use pre-treated SA of soybean seedlings to determine if SA could reduce the damages of plants caused by MSO. By applying MSO to soybean seeds, there are no effects on budding of them. But the growth and development of roots and shoots are inhibited by MSO. GS activity of roots, shoots and cotyledons decrease by increasing MSO concentration. But the GS protein is not only decreased but also accumulated with increasing MSO concentration. This is may be that MSO is converted to MSO-phosphate which mimics the te