奶牛乳房炎致病菌分离鉴定及快速诊断试剂盒的研发和应用.pdfVIP

ISOLATION AND IDENTIFICATION OF BOVINE MASTITIS PATHOGENIC BACTERIA AND THE DEVELOPMENT AND APPLICATION OF RAPID DIAGNOSTIC KIT ABSTRACT In this study, 75 milk samples from 5 different dairy farms in Guangxi were collected. According to the result of bacteria identification, we developed the method of multiple-PCR for mastitis diagnosis, which targeting at Streptococcus agalactiae, staphylococcus aureus, escherichia coli and bacillus cereus. By means of optimizing and assessment, we developed a rapid diagnostic kit based on multiple-PCR which could be clinically used in milk samples and milk products. The experiment results were organized as follows: 1. In total 135 strains of bacteria were found by isolation and identification of 75 milk samples. Among those, we got 24 strains of staphylococcus aureus, 17.87%. 8 strains of staphylococcus epidermidis, 5.93%. 5 strains of staphylococcus saprophyticus, 3.70%. 17 strains of Streptococcus agalactiae,12.59%. 9 strains of streptococcus uberis,6.67%. 7 strains of Streptococcus lactis, 5.19%. 5 strains of streptococcus III dysgalactiae,5.19%. 5 strains of streptococcus faecium , 3.70%. 12 strains of Candida albicans,8.89%.6 strains of saccharomycetes, 4.44%. 17 strains of escherichia coli, 12.59%. 6 strains of Salmonella enteritidis, 4.44%. 2 strains of Shiga bacillus,1.48%. 2 strains of Corynebacterium pyogenes . 1.48%. 10 strains of bacillus cereus, 7.42%. The above data indicated that Streptococcus agalactiae, staphylococcus aureus, escherichia coli and bacillus cereus were the primary pathogenic bacteria. Bovine mastitis in investigated farms were occurr