布渣叶总黄酮基本理化性质研究.pdfVIP

布渣叶总黄酮基本理化性质研究 徐文杰，孙冬梅* （广东省中医研究所，广东广州 510095 ） 摘要：目的：考察布渣叶总黄酮固形物质量、总黄酮提取物的溶解度，研究温度、湿度、 强光照射等对其稳定性的影响。方法：以总黄酮、牡荆苷、异牡荆苷、水仙苷为指标，采用 摇瓶法测定布渣叶总黄酮提取物在水、0.1 mol·L-1盐酸溶液和pH6.8磷酸盐缓冲液（PBS ）中 的表观溶解度与平衡溶解度，并考察光照、温度、湿度对其稳定性的影响。结果：布渣叶总 黄酮提取物表观溶解度及总黄酮的平衡溶解度在0.1 mol·L-1 、盐酸溶液、水和pH6.8 PBS 中均 属于略溶；以牡荆苷、异牡荆苷为指标，布渣叶总黄酮提取物在0.1 mol·L-1盐酸溶液、水和 pH6.8 PBS 中平衡溶解度均为几乎不溶；以水仙苷为指标，布渣叶总黄酮提取物在0.1 mol·L-1 盐酸溶液、水和pH6.8 PBS 中平衡溶解度均为极微溶解。稳定性试验结果显示光照、高温、 高湿条件对布渣叶总黄酮提取物的含量几乎无影响。结论：随着介质的pH增大，布渣叶总 黄酮提取物及其指标成分的溶解度减小。在高湿高温强光照射条件下，布渣叶总黄酮提取物 的4个指标成分均较为稳定。研究结果为布渣叶总黄酮提取物的剂型设计以及其他药学研究 提供实验依据。 关键词：布渣叶；总黄酮；平衡溶解度；稳定性 Study on Base Physicochemical Properties of Total Flavonids of Microtis Folium Extract * XU Wenjie ，SUN Dongmei （Guangdong Institute of Chinese Medicine, Guangzhou 510095, Guangdong, China) Abstract: Objective: To determine the solubility of the Microtis Folium flavonoids extract. The stability of the extract was investigated under high temperature, high humidity and bright light conditions. Methods: Contents of total flavonoids, vitexin, isovitexin and narcissin were chosen as indices. The apparent solubility and equilibrium solubility of Microtis Folium -1 flavonoids extract in water, 0.1 mol • L HCl solution and pH=6.8 phosphate buffer (PBS) were measured by the shake-flask method and its stability was investigated under bright light, high temperature and high humidity conditions. Results: