野生关黄柏中生物碱及绿原酸含量区域性特征剖析.docVIP

野生关黄柏中生物碱及绿原酸含量区域性特征剖析 　　[摘要]该研究在关黄柏基源植物黄檗的整个分布区内，以每一个纬度为间隔设置采样点，在控制采样植株茎粗、采样部位及采样时间的前提下，于31个样点共采集674份野生关黄柏药材样本。对样本中盐酸巴马汀、盐酸小檗碱、盐酸黄柏碱、盐酸药根碱、木兰花碱和绿原酸等6种活性成分的含量进行测定发现，药材中6种活性成分的含量因采样点的不同存在显著差异，辽宁地区所产药材6种活性成分含量最高，其次为北京和吉林地区，黑龙江地区药材的含量最低，研究结果对探究关黄柏药材活性成分形成的环境因素及人工种植区划具有重要的指导意义。 　　[关键词]关黄柏；黄檗；活性成分；区域性特征；差异性分析；聚类分析 　　[Abstract]In the present research， 674 wild medicinal material samples of Phellodendri amurensis Cortex were collected from 31 sampling sites in the whole distribution of its original plant Phellodendron amurense The samples were collected under the premise that the stem diameter of sampling plant， sampling position and time were controlled And the sampling sites were set at the interval of a latitude The content of 6 kinds of active ingredients， palmatine chloride， berberine hydrochloride， phellodendrine chloride， jatrorrhizine hydrochloride， magnoflorine， chlorogenic acid， etc in the medicinal material samples were determined， and the results showed that the content of most active ingredients in the medicinal materials showed significant differences due to the difference of sampling sites Among them， the medicinal materials from Liaoning region had the highest content of active ingredients， followed by Beijing and Jilin regions， and that from Heilongjiang region had the lowest content The study has important directive significance to the exploration of environmental factors for the formation of active constituent and artificial planting regionalization of high quality Phellodendri amurensis Cortex 　　[Key words]Phellodendri Amurensis Cortex； Phellodendron amurense； active constituent； regional characteristic； difference analysis； clustering analysis 　　doi：10.4268/cjcm 　　黄檗的干燥树皮，除去粗皮晒干后为我国传统常用中药关黄柏[1]，关黄柏常用于清热燥湿，泻火除蒸，解毒疗疮；生物碱作为黄檗主要的次生代谢产物，被认为是药材关黄柏的重要活性成分，具有抗炎，抗肿瘤，抗过敏和降血糖等药理活性[24]。作为常用药材，关黄柏是多种中成药的原料药，市场需求很大。由于生存环境恶化，对关黄柏的需求增加以及黄檗自我更新障碍影响资源恢复等原因的长期存在，黄檗野生资源急剧减少，分布范围越来越小。黄檗已被列为重点保护野生药材和国家Ⅱ级保护物种[56]，野生资源受国家法律保护，目前国内市场对关黄柏药材的需求主要依靠进口[79]，关黄柏药材的长期