5 氟尿嘧啶联合奥沙利铂对SW480细胞中Galectin 3表达的影响.docx

优秀毕业论文 精品参考文献资料 目录 中文摘要···················································1 英 文摘要···················································3 前言 ·························································5 正文 ·························································7 材 料与方法··············································7 结果······················································12 讨论 ······················································16 参考文献 ···············································18 综述 ························································21 PAGE 1 PAGE 1 5-氟尿嘧啶联合奥沙利铂对 SW480 细胞中 Galectin-3 表达的影响 华中科技大学同济医学院附属同济医院胃肠外科 硕士研究生：支国俊 硕士生导师：严群 教授 中文摘要 目的：通过研究不同化疗方案对 SW480 细胞中 Galectin-3 表达的影响，探讨化疗药物 对大肠肿瘤的作用及其与 galectin-3 在肿瘤中表达的关系。 方法：以 SW480 细胞建立裸鼠移植瘤模型，随机分成四组：5-氟尿嘧啶组，奥沙利铂 组，联合组（5-氟尿嘧啶联合奥沙利铂）和生理盐水对照组。动态观察四组裸鼠移植 瘤的生长情况，并通过免疫组织化学检测，比较 galectin-3 在各组中的表达差异。 结果：各给药组裸鼠移植瘤的生长速度相对于对照组明显减缓，移植瘤重量明显减轻 （P0.05），而且，联合组抗肿瘤效果相对于单独给药组更加明显（P0.01）。免疫组 织化学结果显示在 5-氟尿嘧啶组、奥沙利铂组及联合组的肿瘤组织中 galectin-3 表达 均减弱，其中，联合组的 galectin-3 表达降低较其他各组更加明显（P0.01）。 结论：5-氟尿嘧啶和奥沙利铂均可抑制肿瘤细胞生长并降低肿瘤组织中 galectin-3 的表 达，二者联合应用抑瘤作用更加明显，对 galectin-3 表达的抑制作用也更加显著。5- 氟尿嘧啶和奥沙利铂对肿瘤细胞的作用与 galectin-3 的表达存在相关性。 关键词：5-氟尿嘧啶；奥沙利铂；galectin-3；SW480 Effects of 5-Fu Combined with Oxaliplatin in Galectin-3 Expression in SW480 Colon Cancer Cells Candidate:Zhi Guojun Major: General Surgery Supervisor: Prof . Yan Qun Department of Gastrointestinal Surgery , Tongji Hospital , Tongji Medical Collage . Huazhong University of Science and Technology , Wuhan 430030 , Chian Abstract Objective: Through the research of galectin-3 expression in tumour cells, to study the effect of 5 – fluorouracil combined with oxaliplatin in the expression of galectin-3 in the tumor tissues. Indirect to study the relaship between the effect of chemotherapy on colorectal cancer and the function of galectin-3 in tumor Methods: Human colon cancer xenografts in nude mice were established by using SW480 cells ,5-Fu group , O xaliplatin group , 5-Fu+Oxaliplatin group