丹皮酚对血管成形术后内膜增生及血管重塑的影响-心血管内科学专业论文.docx

广州医科大学硕士学位论文丹皮酚 广州医科大学硕士学位论文 丹皮酚对血管成形术后内膜增生及血管重塑的影响 - - PAGE 2 - 生减少，差异均有统计学意义(P0.05)。两组 Pae 组(高 Pae 组与低 Pae 组)与缬沙 坦组相比，上述各指标有统计学差异（P0.05）。中膜面积，两组 Pae 组与模型 组相比，无明显统计学差异（P0.05）。两组 Pae 组与缬沙坦组相比，有明显统 计学意义(P0.05)。低 Pae 组与高 Pae 组相比，二者差异无明显统计学差异(P0.05)。 同时各用药组 LA 变化值较对应 IA 变化值明显增大，差异有统计学意义(P0.05)。 2.TUNEL 染色细胞凋亡图像分析结果：模型组凋亡细胞散在分布，数量较少， 用药组与模型组相比，凋亡细胞明显增多,多集中于血管内膜，差异有统计学意 义(P0.05)。但低 Pae 组与高 Pae 组相比，二者无明显统计学差异(P0.05)，同时 两组 Pae 组与缬沙坦组相比，差异有统计学意义（P0.05）。 3.mmp-2、bcl-2 免疫组化图像分析：模型组 mmp-2 及 bcl-2 阳性细胞主集中于内 膜靠近管腔侧,分布较多，用药组分别与模型组相比，阳性细胞分布明显减少,差 异有统计学意义（P0.05）。但低 Pae 组与高 Pae 组相比，二者无明显统计学差 异（P0.05），同时两组 Pae 组与缬沙坦组相比，有统计学差异（P0.05）。 结论： 1. 丹皮酚通过抑制胶原的过度沉积，抑制PTA术后内膜增厚及血管重塑的发生。 2. 丹皮酚通过抑制PTA术后mmp-2的表达，抑制PTA术后血管重塑的发生。 3.丹皮酚通过抑制bcl-2的过度表达而促进细胞凋亡，从而抑制PTA术后内膜增生 及血管收缩性重塑发生。 关键词：丹皮酚 血管成形术 再狭窄 细胞凋亡 Effect of Paeonol on intimal hyperplasia and vascular remodeling after angioplasty M.S Candidate:Bai Li Supervisor:Prof.Chen Cibin Major: Cardiovascular Medicine Abstract Purpose: observe the effect of paeonol (Pae)on the model of vascular restenosis after angioplasty.Firstly,we established the model of atherosclerosis by giving rabbits high fat diet and injurying intima of aorta abdominalis with balloon.Secondly,we established the model of vascular restenosis after angioplasty by balloon dilation.Investigate and discuss the mechanism of Pae preventing restenosis after angioplasty. Material and Method: choose 40 male adult New Zealand white rabbits weighting from 2.5 to 3.0kg.Select randomly 6 rabbits which were fed standard diet as Sham operation group. each rabbit of the rest was fed high fat diet every day for 8 weeks and to injury its intima of aorta abdominalis with balloon,we established the model of atherosclerosis.8 weeks later select randomly 6 rabbits in the model of atherosclerosis group to kill and draw materials,compared with 6 rabbits in Sham operation group to prove the model of atherosclerosis was success. then we established the model of vascular restenosis after angioplasty by ex