除草剂丁草胺对花背蟾蜍胚后早期脑部发育的毒性作用-神经生物学专业论文.docxVIP

and morphology of tadpoles were changed significantly, showing obvious diapauses effect, and the effect on body weight compared with on body length was more obvious; The morphology of the neurons in the tadpole brain were nearly not affected by acute or chronic exposure, but the exposure made the ventricle ependymal produce a protective reaction to rise its thickness; In the early period of exposure, the number of the neurons decreased, especially for the neurons in medulla oblongata, but followed the adaption of their own bodies, the number of neurons returned to normal levels again. Keywords Bufo naddei Strauch; early period of postembryonic development; herbicide; butachlor; brain 目 录 HYPERLINK \l _bookmark0 第一章 综 述 1 HYPERLINK \l _bookmark1 1.1 引言 1 HYPERLINK \l _bookmark2 1.2 丁草胺的研究概况1 HYPERLINK \l _bookmark3 1.2.1 丁草胺的理化性质及其作用机理1 HYPERLINK \l _bookmark4 1.2.2 丁草胺的应用和降解残留2 HYPERLINK \l _bookmark5 1.2.3 丁草胺的毒性研究3 HYPERLINK \l _bookmark6 1.2.3.1 丁草胺的致死浓度和致死机制3 HYPERLINK \l _bookmark7 1.2.3.2 丁草胺对动物生长发育的影响4 HYPERLINK \l _bookmark8 1.2.3.3 丁草胺的遗传毒性和对酶活性的影响5 HYPERLINK \l _bookmark9 1.3 常见化学物质及农药的神经毒性研究进展6 HYPERLINK \l _bookmark10 1.3.1 常见化学物质的神经毒性作用6 HYPERLINK \l _bookmark11 1.3.2 常见农药的神经毒性作用6 HYPERLINK \l _bookmark12 1.4 两栖类动物对环境安全的意义7 HYPERLINK \l _bookmark13 1.5 论文立题依据、内容及意义8 HYPERLINK \l _bookmark14 1.6 创新之处 9 HYPERLINK \l _bookmark15 第二章 实验材料与方法10 HYPERLINK \l _bookmark16 2.1 实验动物 10 HYPERLINK \l _bookmark17 2.2 实验药品及试剂 10 HYPERLINK \l _bookmark18 2.3 实验方法 10 HYPERLINK \l _bookmark19 2.3.1 丁草胺对花背蟾蜍的急性毒性实验10 HYPERLINK \l _bookmark20 2.3.2 丁草胺对花背蟾蜍的慢性毒性实验 11 HYPERLINK \l _bookmark21 2.4 石蜡切片及尼氏染色方法12 HYPERLINK \l _bookmark22 2.5 脑神经元计数以及室管膜厚度测量12 HYPERLINK \l _bookmark23 2.6 数据统计分析 13 HYPERLINK \l _bookmark24 第三章 结果与讨论 14 HYPERLINK \l _bookmark25 3.1 丁草胺急性染毒对花背蟾蜍胚后早期的毒性作用14 HYPERLINK \l _bookmark26 3.1.1 染毒后蝌蚪的 LC50 及其 95%置信区间、安全浓度 14 HYPERLINK \l _bookmark27 3.1.2 急性染毒对花背蟾蜍胚后早期形态学的影响14 HYPERLINK \l _bookmark28 3.1.3 急性染毒后花背蟾蜍胚后早期的行为