大劣按蚊PPO基因的克隆与抗约氏疟原虫感染免疫防御反应的研究-病原生物学专业论文.docx

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 中英文缩写一览表 简写 Ad 英文名称 Anopheles dirus 中文名称 大劣按蚊 Ag AMP Blast bp cDNA DEPC DNA dNTP EB EDTA IPTG Kb Anopheles gambiae ampicillin Basic Local Alignment Search Tool base pair complementary deoxyribonucleic acid diethyl pyroocarbonate deoxyribonucleic acid deoxynucleoside triphosphate ethidium bromide ethylenediamineteracetic acid Isopropylthio--D-galactoside kolo-base pair 冈比亚按蚊 氨苄青霉素 局部相似性基本查询工具 碱基对 互补脱氧核糖核酸 焦碳酸二乙脂 脱氧核糖核酸 脱氧三磷酸核苷 溴化乙锭 乙二胺四乙酸 异丙基硫代--D-半乳糖苷 千碱基对 MMLV NBT moloney murine leukemia virus nitro-blue tetrazolium 鼠白血病病毒 硝基四氮唑兰 NO OD Oligo PBS PCR PPO RACE RNA RNase rpm RT-PCR SDS nitrogen monoxide optical density oligonucletide phosphate buffered saline polymerase chain reaction prophenoloxidase rapid amplification cDNA ends ribonucleotide acid ribonuclease revolutions per minute reverse transcript polymerase chain reaction laurouyl sulfate 一氧化氮 光密度 寡聚核苷酸 磷酸盐缓冲液 聚合酶链式反应 前酚氧化酶 快速扩增 cDNA 未端 核糖核酸 RNA 酶 转/分钟 逆转录 PCR 十二烷基硫酸钠 Taq therus aquaticus DNA (polymerase) 耐热 DNA 聚合酶 Tris Tris-(hydroxymethyl)-aminomethane 三羟甲基氨基甲烷 U unit 单位 X-gal 5-bromo-4-chloro-3-indolyl--D-galactoside 5-溴-4-氯-3-吲哚- -D-半 乳糖苷 氨基酸缩写 A C D Ala Cys Asp alanine cysteine aspatic aci E F G H I Glu Phe Gly His Ile glutamic ac henylalan glycine histidine isoleucine K L M N P Lys Leu Met Asn Pro lysine leucine methionin asparagine proline Q R S T V Gln Arg Ser Thr Val glutamine arginine serine threonine valine W Y Trp Tyr tryptopha tyrosine Cloning of prophenoloxidase gene of Anopheles dirus and study on the immune defence reaction against Plasmodium yoelii infection Abstract Malaria, transmitted by a mosquito vector,is a severe infectious disease with high incidence rate and fatality rate.Control of malaria will be confronted with great challenge because of the rapid spread of multi-drug resistant parasites,the emergence of insecticide-resistant anopheline mosquitoes and the lack of effective vaccines fighting against malaria,which have emphasized the need for