芳香二肽与一价金属离子间相互作用及DNA切割活性的分析-有机化学专业论文.docxVIP

摘要 摘要 万方数据 万方数据 的定性研究。研究结果显示，同时加入二肽和 Co2+离子后，DNA 的 CD 光谱吸 收曲线在 246 nm 和 275 nm 处的正负吸收峰均有所增强。由此我们推测二肽在 金属离子辅助下与遗传物质 DNA 作用方式主要是沟面结合，但同时也存在部分 的插入作用。 利用高效液相色谱法(HPLC)测定了十种芳香二肽与 Li+、Na+、Cs+、K+、 Ag+几种一价金属离子的结合常数。实验结果表明，当芳香二肽相同时，不同的 一价金属离子中过渡金属 Ag+与二肽的作用力最强；相同的金属离子与十种芳香 二肽作用时，L-组氨酸-L-组氨酸二肽与金属离子的结合能力最强。 关键词：芳香二肽；DNA 切割；金属离子；作用方式；结合常数 II Abst Abstract Abstract Peptides, a kind of biological molecules is made up of amino acid unit, widely exist in nature and exhibit a large number of fascinating biological activities. As the smallest molecular in peptides, dipeptide is found that has diverse biological activities, such as antibiotic, antibacterial, antivirus, anticancer, immunity regulation and ion carrier. Moreover, the oxidation resistance and antimicrobial properties of some small peptide could be improved by combining with metal ions; at the same time, metal ions can also improve its bioavailability. The research on metal ion-protein interaction has been an important content of life science researches. Metal ions interact with protein using a great variety of mechanisms to regulate protein functions. Because of the structure complexities of proteins, it is extremely difficult to investigate directly. It has been common practice to use dipeptides as model compounds since they make up of the protein structures. HYPERLINK /so/ So there is important theoretical significance and academic value to carry out HYPERLINK /research/ researches on molecular recognition of dipeptides with metal ions. In this dissertation, the cleavage activity of 10 aromatic linear dipeptides to supercoiled pUC19 DNA was investigated in the presence of Cu(II), Zn(II), Co(II), Ca(II) and Mg(II) by agarose gel electrophoresis. The experimental results indicate that aromatic dipeptides had hardly any cleavage activities by the absent metal ions， and the cleavage activities could markedly enhanced when Co(II) was added to the solution. The optimum conditions on the cleavage of pUC19 DNA by dipeptides an