雌激素诱导结肠癌细胞凋亡的表观遗传机制研究-内科学(消化系病)专业论文.docxVIP

第三军医大学博士学位论文 第三军医大学博士学位论文 PAGE PAGE 10 H Hour 小时 IM Invert microscope 倒置显微镜 M Mole per liter 摩尔每升 mg Milligram 毫克 min Minute 分钟 mL Millilite 毫升 MMR Mismatch repair 错配修复 mRNA Messenger RNA 信使 RNA MSI Microsatellite instability 微卫星不稳定性 OD Optical density 光密度 PAGE Polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PBS Phosphate-buffer saline 磷酸盐缓冲液 PCR Polymerase chain reaction 聚合酶链式反应 PI Positivity index 阳性指数 PVDF Polyvinylidene difluoride 聚偏(二)氟乙烯 RIPA radio immunoprecipition assay 放射免疫沉淀细胞裂解液 RNA Ribonucleic acid 核糖核酸 RNase Ribonuclease RNA酶 RPM Round per minute 转/分 RT-PCR Reverse transcriptase PCR 反转录PCR RTQ-PCR Real-time quantitative polymerase chain reaction 实时定量聚合酶链反应 Rrna Ribosomal ribonucleic acid 核蛋白体核糖核酸 S Second 秒 SD Standard deviation 标准差 SDS Sodium Dodecyl Sulfate 十二烷基硫酸钠 SDS- PAGE  Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis 十二烷基硫酸钠聚丙烯酰 胺凝胶电泳 TAE Tris-Acetate-EDTA 乙酸电泳缓冲液 Taq Thermus aquaticus DNA Polymerase 栖热水生菌DNA聚合酶 TBE Tris-Borate-EDTA 硼酸电泳缓冲液 TBS Tris-buffered saline Tris-HCl缓冲盐溶液 TBST Tris Buffered Saline with Tween 20 含20%Tween20TBS 缓冲液 TE Tris-EDTA TE缓冲液 TEMED tetramethylethylenediamine 四甲基乙二胺 Tris Tris (hydroxymethyl) aminomethane 三(羟甲基)氨基甲烷 U Unit 单位 μg Microgram 微克 μL Microliter 微升 μm Micrometer 微米 V Voltage 伏特 WB Western Blot 蛋白免疫印迹杂交 The Study of Epigenetic Mechanism of Estrogen Inducing CRC Cells Apoptosis Abstract Background Colorectal cancer (CRC) is the fourth most common cause of death from cancer, affects around 1.2 million people world wide, with about 608,700 fatalities per year. Both the prevalence and the lifetime risk of CRC are significantly lower in females than in males. This is particularly true when comparing pre-menopausal women to age-matched men. The sex-specific difference in CRC incidence has been attributed to estrogen, but the exact mechanism is not clear. Since expression alterations of some miRNAs are associated with a series of genetic events, their expression patterns may either have pro- or anti- ca