定量检测阿尔茨海默病模型小鼠海马代谢物浓度的研究.docx

优秀毕业论文 精品参考文献资料 中文摘要 目的： 通过应用 9.4T 液态离体型核磁共振频谱仪（Bruker Avance 400MHz）对阿尔茨 海默病模型小鼠海马代谢物的浓度变化进行分析。 方法： 20 只昆明小鼠雌雄各半，随机分成实验组和对照组，实验组小鼠连续 60 天颈背 部皮下注射 D-半乳糖 120mg/（kg.d）和亚硝酸钠 90mg/（kg.d），对照组注射相同剂量的 生理盐水。打药 24 小时后所有小鼠进行 Morris 水迷宫行为学检测，之后均采用断头牺牲 ， 取右侧大脑半球分离海马，运用组织高氯酸萃取法，提取小鼠海马组织内代谢物，进行 9.4T 液态离体型高分辨率核磁共振扫描，所得数据线经 XWINNMR(Bruker GmBH)软件初步处理， 再应用 MestRe-C4.3 核磁共振专用软件进行综合分析处理；左侧取后半脑作病理组织学检 测。 结果： 对照组及模型组小鼠海马代谢浓度分别为NAA：43.63±7.67 mmol/L and 34.66 ±6.79 mmol/L; Cho：53.09±4.32 mmol/L and 48.62±7.92 mmol/L;Glu：26.87±5.46 mmol/L and 14.87 ± 2.68 mmol/L;mI ： 45.93 ± 6.73 mmol/L and 74.09 ± 8.09 mmol/L,the differences of two groups were statistically significance。其中两组 间各代谢物浓度的差别具有统计学意义（P0.05)。Morris水迷宫试验对照组和模型组逃 避潜伏期、目标象限游泳时间及距离百分比有明显差异（P0.05)。在400倍光镜下，HE染 色模型组小鼠海马神经细胞层次明显减少，细胞排列稀疏，细胞核深染。改良Bielschowsky 染色可见神经元排列散乱，大脑皮层及海马区可见较多胞体和轴突染色较深的神经元并成 拖尾形状，形成神经元纤维缠结。刚果红染色可见桔红色沉积物，呈斑片状。获得的高质 量氢质子频谱，常规的海马代谢物被探测到并被定位。 结论： D-半乳糖联合亚硝酸钠能导致小鼠成为阿尔茨海默病，其海马 NAA、Cho、Glu 的 浓度较对照组明显降低，mI 有升高的趋势。 关键词： 阿尔茨海默病；海马；脑代谢物；离体 1H-MRS；定量分析 PAGE PAGE 3 Abstract AIM： To study Alzheimers disease mice caused by the D-galactose and NaNO2 ,observe the alteration of hippocampal metabolites using in vitro 9.4T high resolution magnetic resonance spectroscopy. METHODS ： Twenty kunming mice were randomly divided into two groups, model group and control group. Female to male ratio was 1:1. The mice in the model group were subcutaneouly injected with D-galactose 120 m g /（kg.d）, NaNO2 90 mg/ （ kg.d ） for 60 days and the control group with oral saline.24 hours later after Morris water maze test ， all mice were sacrificed and the right hippocampus were dissected for 1H-MRS examination. Data were collected using in vitro 9.4 T high resolution magnetic resonance spectrometer. Spectra were processed using XWINNMR and MestRe-c 4.3. Compared with the left side，HE and Bielschowsky silver impregnation and congored coloration were employed to detect and confirm the change of brain cells. RESULTS ： Good 1H-MR spect